A novel lanthanide-chelate based molecularly imprinted cryogel for purification of hemoglobin from blood serum: An alternative method for thalassemia diagnosis

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dc.contributor.authorDolak, Ibrahim
dc.contributor.authorCanpolat, Gurbet
dc.contributor.authorOnat, Ruken
dc.contributor.authorKecili, Rustem
dc.contributor.authorBaysal, Zubeyde
dc.contributor.authorZiyadanogullari, Berrin
dc.contributor.authorErsoz, Arzu
dc.date.accessioned2024-12-24T19:27:33Z
dc.date.available2024-12-24T19:27:33Z
dc.date.issued2020
dc.departmentSiirt Üniversitesi
dc.description.abstractPurification and analyzing of proteins is an essential means for understanding their function and diseases associated with their lack or defect. In this research, a new lanthanide-chelate based molecularly imprinted polymer (MIP) was synthesized for selective separation of Hemoglobin (Hb) from human serum in the presence of various interference molecules. The Hb-imprinted polymer was prepared by using complex functional monomer N-methacryloylamido antipyrine (MAAP)-Ce(III) and 2-Hydroxyethyl methacrylate (HEMA) in accordance of cryopolymerization techniques. The nonimprinted cryogel (NIP) was also prepared at same polymerization conditions in the absence of template Hb molecule. The effects of pH, initial Hb concentration, flow rate, temperature and ionic strength on the binding capacity of both imprinted and nonimprinted cryogels was investigated. The maximum binding capacity for the MIP column was found to be as 79.41 mg g(-1) dry cryogel, that is four times higher than the NIP column under the optimum conditions (pH 5.0, flow rate: 1.0 mL min(-1), T: 25 degrees C). Moreover, selectivity experiments were performed by using two interference proteins as myoglobin (Mb) and cytochrome c (Cyt-c) and the relative selectivity coefficients (k') for Hb/Mb and Hb/Cyt-c pairs were determined as 36.59 and 37.22, respectively.
dc.description.sponsorshipDicle University BAP [FEN.17.001]
dc.description.sponsorshipThe authors would like to thank the Dicle University BAP for the financial support (Proj. No. FEN.17.001).
dc.identifier.doi10.1016/j.procbio.2019.12.011
dc.identifier.endpage196
dc.identifier.issn1359-5113
dc.identifier.issn1873-3298
dc.identifier.scopus2-s2.0-85077356761
dc.identifier.scopusqualityQ1
dc.identifier.startpage189
dc.identifier.urihttps://doi.org/10.1016/j.procbio.2019.12.011
dc.identifier.urihttps://hdl.handle.net/20.500.12604/6698
dc.identifier.volume91
dc.identifier.wosWOS:000526117900022
dc.identifier.wosqualityQ2
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.language.isoen
dc.publisherElsevier Sci Ltd
dc.relation.ispartofProcess Biochemistry
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.snmzKA_20241222
dc.subjectMolecularly imprinted cryogels
dc.subjectLanthanide chelate
dc.subjectHemoglobin
dc.subjectSelective separation
dc.subjectThalassemia
dc.titleA novel lanthanide-chelate based molecularly imprinted cryogel for purification of hemoglobin from blood serum: An alternative method for thalassemia diagnosis
dc.typeArticle

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