Preliminary investigation of the prevalence and genotype distribution of Cryptosporidium spp., and Giardia duodenalis in cats in Siirt, Turkey

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dc.authoridAkyildiz, Gurkan/0000-0002-8610-5174
dc.contributor.authorAslan Celik, Burcak
dc.contributor.authorCelik, Ozgur Yasar
dc.contributor.authorAyan, Adnan
dc.contributor.authorAkyildiz, Gurkan
dc.contributor.authorOrunc Kilinc, Ozlem
dc.contributor.authorOktay Ayan, Ozge
dc.contributor.authorErcan, Kerem
dc.date.accessioned2024-12-24T19:30:47Z
dc.date.available2024-12-24T19:30:47Z
dc.date.issued2023
dc.departmentSiirt Üniversitesi
dc.description.abstractCryptosporidium spp. and Giardia duodenalis are protozoan parasites found in humans and many animal species worldwide. The aim of this study was to determine the prevalence and genotypes of Cryptosporidium spp. and Giardia duodenalis in cats and to evaluate the zoonotic potential of these agents. The animal material of the study consisted of a total of 40 cats brought to the Veterinary Faculty. Fresh fecal samples taken from the cats were placed in individual sample containers. All samples were examined under the microscope by Kinyoun Acid Fast staining for Cryptosporidium spp. and by the native-Lugol method for Giardia duodenalis. Nested PCR and sequence analyses were then performed. As a result of microscopic and nested PCR analyses for Cryptosporidium spp., no positivity was found in any sample. The prevalence of Giardia duodenalis was 2.5% in both microscopic examination and nested PCR analyses. When the DNA sequences of the beta-Giardin gene obtained in the study were compared with the database in NCBI Basic Local Alignment Search Tool, it was determined that one sample overlapped with Assemblage B samples. As a result of this study, the prevalence of Cryptosporidium spp. and Giardia duodenalis in cats was determined and the presence of Assemblage B was revealed. It is recommended that repetitive studies should be carried out as much as possible to determine the possible role of these parasites in the transmission of these parasites to humans.
dc.identifier.doi10.2478/acve-2023-0024
dc.identifier.endpage324
dc.identifier.issn0567-8315
dc.identifier.issn1820-7448
dc.identifier.issue3
dc.identifier.scopus2-s2.0-85173483816
dc.identifier.scopusqualityQ3
dc.identifier.startpage317
dc.identifier.urihttps://doi.org/10.2478/acve-2023-0024
dc.identifier.urihttps://hdl.handle.net/20.500.12604/7684
dc.identifier.volume73
dc.identifier.wosWOS:001077026100002
dc.identifier.wosqualityQ3
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.language.isoen
dc.publisherSciendo
dc.relation.ispartofActa Veterinaria-Beograd
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.snmzKA_20241222
dc.subjectNested PCR
dc.subjectSequence
dc.subjectPhylogeny
dc.titlePreliminary investigation of the prevalence and genotype distribution of Cryptosporidium spp., and Giardia duodenalis in cats in Siirt, Turkey
dc.typeArticle

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