Analysis of genetic diversity and population structure of Iranian Artemisia annua L. accessions using IRAP-based molecular markers and revealing retroelement insertional polymorphism

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dc.contributor.authorValizadeh, Negar
dc.contributor.authorHolasou, Hossein Abbasi
dc.contributor.authorMohammadi, Seyyed Abolghasem
dc.contributor.authorAgamirzaoglu, Muhsin
dc.date.accessioned2024-12-24T19:24:37Z
dc.date.available2024-12-24T19:24:37Z
dc.date.issued2024
dc.departmentSiirt Üniversitesi
dc.description.abstractRetrotransposons (RTNs) are a primary source of structural evolution in plant genomes, propagating through a copy-and-paste mechanism. This makes them widely utilized in plants as effective molecular markers for DNA fingerprinting, genetic mapping, and assessing genetic variability. Inter-retrotransposon amplified polymorphisms (IRAPs) were employed to evaluate genetic variability and structure in a collection of 118 Artemisia annua (sweet wormwood) accessions, identifying and amplifying 849 loci with 32 IRAP primers derived from Rosaceae, Gramineae, and Solanaceae retroelements. The single IRAP primer Tnt1.OL16, based on RTNs, produced the highest number of markers. The percentage of polymorphic loci (PPL), mean expected heterozygosity (He), number of effective alleles (Ne), and Shannon's information index (I) in the studied collection were 95.80%, 0.30, 1.48, and 0.46, respectively. Although the analysis of molecular variance (AMOVA) revealed no significant genetic structure among populations, the four populations clustered into three groups in the neighbor joining (NJ) dendrogram, indicating relatively higher genetic variation within each population. In conclusion, low genetic diversity was detected in Iranian sweet wormwood, suggesting it forms a distinct population ideal for the uniform extraction of artemisinin. Breeding efforts could be enhanced by introducing exotic or improved germplasm to mitigate potential inbreeding depression effects.
dc.description.sponsorshipGenomics & Molecular Breeding Lab, Center of Excellence in Cereal Molecular Breeding, University of Tabriz, Iran; University of Tabriz incorporation with the Iran National Elites Foundation [102-1565]
dc.description.sponsorshipThe authors gratefully acknowledge the laboratory facilities provided by the Genomics & Molecular Breeding Lab, Center of Excellence in Cereal Molecular Breeding, University of Tabriz, Iran. The research was financially supported by the University of Tabriz incorporation with the Iran National Elites Foundation, under vote NO. 102-1565.
dc.identifier.doi10.1007/s10722-024-02261-3
dc.identifier.issn0925-9864
dc.identifier.issn1573-5109
dc.identifier.scopus2-s2.0-85209078671
dc.identifier.scopusqualityQ2
dc.identifier.urihttps://doi.org/10.1007/s10722-024-02261-3
dc.identifier.urihttps://hdl.handle.net/20.500.12604/6054
dc.identifier.wosWOS:001355672200001
dc.identifier.wosqualityN/A
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.language.isoen
dc.publisherSpringer
dc.relation.ispartofGenetic Resources and Crop Evolution
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.snmzKA_20241222
dc.subjectArtemisia annua L
dc.subjectGenetic diversity
dc.subjectLTR Retrotransposon
dc.subjectModel-based cluster
dc.titleAnalysis of genetic diversity and population structure of Iranian Artemisia annua L. accessions using IRAP-based molecular markers and revealing retroelement insertional polymorphism
dc.typeArticle

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