Isolation of a thermophilic Anoxybacillus flavithermus sp nov and production of thermostable ?-amylase under solid-state fermentation (SSF)

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dc.authoridOkumus, Veysi/0000-0002-5505-2700
dc.authoridMatpan Bekler, Fatma/0000-0001-8253-9568
dc.authoridDUNDAR, ABDURRAHMAN/0000-0002-7930-1054
dc.contributor.authorOzdemir, Sadin
dc.contributor.authorMatpan, Fatma
dc.contributor.authorOkumus, Veysi
dc.contributor.authorDundar, Abdurrahman
dc.contributor.authorUlutas, Mehmet Sefa
dc.contributor.authorKumru, Mert
dc.date.accessioned2024-12-24T19:25:04Z
dc.date.available2024-12-24T19:25:04Z
dc.date.issued2012
dc.departmentSiirt Üniversitesi
dc.description.abstractA new bacteria was isolated from hot-spring water of Gazligol, Afyonkarahisar in Turkey. Based on morphological and biochemical tests and 16S rRNA gene sequence analysis, the isolate belonged to the Anoxybacillus flavithermus species which has 99% similarity with the bacterium DNA. The production of alpha-amylase by thermophilic Anoxybacillus flavithermus was investigated under solid-state fermentation by using some agricultural waste as substrates. Solid substrates such as rice husk, banana husk, millet, water melon husk, lentil bran, wheat bran and maize oil cake were studied for enzyme production. Of these, rice husk was proved as the best substrate for alpha-amylase production (1,271 U/mg). The maximum alpha-amylase production was observed as 1,803 U/mg at 72 h, 1,000 mu m particle size, 70% initial moisture content (w/v), and 40% inoculum level (v/w). Among the various nitrogen sources tested, 1% peptone (3,170 U/mg) was found to be the best nitrogen source for alpha-amylase production. As additional carbon sources, 1% starch (2,364 U/mg) enhanced alpha-amylase production. The optimum temperature for the activity of alpha-amylase was found to be 70A degrees C. The enzyme was optimally active at pH 6.0 and stable in the pH range of 6.0-8.0.
dc.identifier.doi10.1007/s13213-011-0385-4
dc.identifier.endpage1375
dc.identifier.issn1590-4261
dc.identifier.issn1869-2044
dc.identifier.issue4
dc.identifier.scopus2-s2.0-84871428842
dc.identifier.scopusqualityQ2
dc.identifier.startpage1367
dc.identifier.urihttps://doi.org/10.1007/s13213-011-0385-4
dc.identifier.urihttps://hdl.handle.net/20.500.12604/6231
dc.identifier.volume62
dc.identifier.wosWOS:000310886200004
dc.identifier.wosqualityQ3
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.language.isoen
dc.publisherBmc
dc.relation.ispartofAnnals of Microbiology
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.snmzKA_20241222
dc.subjectAgro-industrial
dc.subjectalpha-Amylase
dc.subjectSolid-state fermentation (SSF)
dc.subjectThermophilic bacteria
dc.titleIsolation of a thermophilic Anoxybacillus flavithermus sp nov and production of thermostable ?-amylase under solid-state fermentation (SSF)
dc.typeArticle

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