Applications of genome editing in plant virus disease management: CRISPR/Cas9 plays a central role
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Tarih
2023
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Taylor & Francis Inc
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
Plant viruses infect a wide variety of economically important crop plants and cause significant loss in agricultural production around the world. Conventional control strategies are insufficient to combat rapidly evolving plant viruses. In recent years, genome editing technologies have paved new ways for manipulating viral genomes (DNA or RNA). Among them, the Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) and CRISPR-associated protein 9 (Cas9) system has been seen to be able to engineer plant virus resistance by directly targeting the viral genome as well as by inactivating host susceptibility genes. In this review, we survey genome editing tools targeting viral genomes, with an emphasis on CRISPR/Cas9. The advantages of the CRISPR/Cas9 system for combating plant viruses as well as its limitations are discussed in detail.
Açıklama
Anahtar Kelimeler
CRISPR, Cas9, genome editing, gRNA, Mega-nuclease, plant virus, TALEN, virus resistance, ZFN
Kaynak
Canadian Journal of Plant Pathology
WoS Q Değeri
Q3
Scopus Q Değeri
Q2
Cilt
45
Sayı
5-6
