Prevalence and molecular characterization of Cryptosporidium spp. in calves in the Siirt Province, Turkiye

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dc.authoridGormez, Gul/0000-0001-6980-4988
dc.authoridAkyildiz, Gurkan/0000-0002-8610-5174
dc.contributor.authorCelik, Ozgur Yasar
dc.contributor.authorSahin, Tekin
dc.contributor.authorCelik, Burcak Aslan
dc.contributor.authorKilinc, Ozlem Orunc
dc.contributor.authorAyan, Adnan
dc.contributor.authorAkyildiz, Gurkan
dc.contributor.authorAyan, Ozge Oktay
dc.date.accessioned2024-12-24T19:30:50Z
dc.date.available2024-12-24T19:30:50Z
dc.date.issued2023
dc.departmentSiirt Üniversitesi
dc.description.abstractCryptosporidiosis, one of the main protozoan infections of the last century, is especially dangerous for calves and causes significant economic losses. This research was carried out to determine the prevalence of Cryptosporidium spp. by microscopic and molecular methods and to determine subtypes in 100 calves up to 6 months old and with diarrhoea in the Siirt Province, Turkiye. As a result of the microscopic examination (Kinyoun's acid-fast), Cryptosporidium spp. oocysts were found in 8 (8%) of 100 samples. As a result of nested PCR, 826-864 bp specific bands for Cryptosporidium spp. were obtained in 13 (13%) of 100 samples. When the DNA sequences of the SSU rRNA gene were compared with the NCBI Basic Local Alignment Search Tool database, it was determined that eight samples sequence analyses showed 100% similarity with the C. parvum, C. ryanae, and C. bovis samples. The detection of C. parvum, which has zoonotic importance in this study, suggests that calves with diarrhoea may be a source of contamination for other animals and humans. Therefore, animal owners and people in close contact with animals should be informed about the public health of cryptosporidiosis.
dc.description.sponsorshipScientific Research Projects Coordinatorship of the Siirt University [2021-SIUVET-08]
dc.description.sponsorshipThis research was supported by the Scientific Research Projects Coordinatorship of the Siirt University within the scope of the project no. 2021-SIUVET-08.
dc.identifier.doi10.2754/avb202392030243
dc.identifier.endpage+
dc.identifier.issn0001-7213
dc.identifier.issn1801-7576
dc.identifier.issue3
dc.identifier.scopus2-s2.0-85168779487
dc.identifier.scopusqualityQ3
dc.identifier.startpage243
dc.identifier.urihttps://doi.org/10.2754/avb202392030243
dc.identifier.urihttps://hdl.handle.net/20.500.12604/7705
dc.identifier.volume92
dc.identifier.wosWOS:001153359000003
dc.identifier.wosqualityQ3
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.language.isoen
dc.publisherVeterinarni A Farmaceuticka Univerzita Brno
dc.relation.ispartofActa Veterinaria Brno
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.snmzKA_20241222
dc.subjectCattle
dc.subjectC. parvum
dc.subjectC. ryanae
dc.subjectC. bovis
dc.subjectnested PCR
dc.titlePrevalence and molecular characterization of Cryptosporidium spp. in calves in the Siirt Province, Turkiye
dc.typeArticle

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