Detection and molecular characterization of mastadenoviruses in calves with respiratory system infection

Basit Öğe Kaydı
dc.contributor.authorHacıoğlu, İlke Karayel
dc.contributor.authorAlkan, Feray
dc.contributor.authorYelken, Selda Duran
dc.date.accessioned2024-12-24T19:16:03Z
dc.date.available2024-12-24T19:16:03Z
dc.date.issued2022
dc.departmentSiirt Üniversitesi
dc.description.abstractAim: Bovine adenovirus (BAdV) is one of the viral agents that causes respiratory illness in cattle, along with numerous other viral agents. Although the prevalence of BAdV has been determined by serological studies conducted in our\rcountry, there are limited data on the detection of BAdVs in respiratory system\rsamples, and there is no study on their molecular characterization. The aim of\rthis study was to detect and characterize BAdVs of the Mastadenovirus genus.\rMaterials and Methods: In this study, a total of 64 nasal swabs and one\rlung sample from 65 cattle of different ages with respiratory system disease\rsymptoms were used. After the extraction of viral DNA, they were tested by\rPCR using primers targeting the hexon gene region and the expected size of\ramplicons were sequenced.\rResults: According to the PCR results, three samples were found positive and\rthe positivity rate was detected as 4.6%. Out of positive samples, two were\rable to be sequenced and one clustered in the BAdV-2 serotype while the other\rwas grouped in the BAdV-3 serotype.\rConclusion: In this study, it was revealed that BAdVs are contributing agents\rin respiratory system infection in our country. Also, this is the first study reporting the molecular characterization of BAdV-2 and BAdV-3 in calves with\rrespiratory system infection in Turkey.
dc.identifier.doi10.15312/EurasianJVetSci.2022.368
dc.identifier.endpage89
dc.identifier.issn1309-6958
dc.identifier.issn2146-1953
dc.identifier.issue2
dc.identifier.startpage83
dc.identifier.trdizinid521605
dc.identifier.urihttps://doi.org/10.15312/EurasianJVetSci.2022.368
dc.identifier.urihttps://search.trdizin.gov.tr/tr/yayin/detay/521605
dc.identifier.urihttps://hdl.handle.net/20.500.12604/4205
dc.identifier.volume38
dc.indekslendigikaynakTR-Dizin
dc.language.isoen
dc.relation.ispartofEurasian Journal of Veterinary Sciences
dc.relation.publicationcategoryMakale - Ulusal Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.snmzKA_20241222
dc.subjectBiyoloji,Solunum Sistemi,Mikrobiyoloji,Veterinerlik
dc.titleDetection and molecular characterization of mastadenoviruses in calves with respiratory system infection
dc.typeArticle

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