Detection and molecular characterization of mastadenoviruses in calves with respiratory system infection
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Tarih
2022
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Aim: Bovine adenovirus (BAdV) is one of the viral agents that causes respiratory illness in cattle, along with numerous other viral agents. Although the prevalence of BAdV has been determined by serological studies conducted in our\rcountry, there are limited data on the detection of BAdVs in respiratory system\rsamples, and there is no study on their molecular characterization. The aim of\rthis study was to detect and characterize BAdVs of the Mastadenovirus genus.\rMaterials and Methods: In this study, a total of 64 nasal swabs and one\rlung sample from 65 cattle of different ages with respiratory system disease\rsymptoms were used. After the extraction of viral DNA, they were tested by\rPCR using primers targeting the hexon gene region and the expected size of\ramplicons were sequenced.\rResults: According to the PCR results, three samples were found positive and\rthe positivity rate was detected as 4.6%. Out of positive samples, two were\rable to be sequenced and one clustered in the BAdV-2 serotype while the other\rwas grouped in the BAdV-3 serotype.\rConclusion: In this study, it was revealed that BAdVs are contributing agents\rin respiratory system infection in our country. Also, this is the first study reporting the molecular characterization of BAdV-2 and BAdV-3 in calves with\rrespiratory system infection in Turkey.
Açıklama
Anahtar Kelimeler
Biyoloji,Solunum Sistemi,Mikrobiyoloji,Veterinerlik
Kaynak
Eurasian Journal of Veterinary Sciences
WoS Q Değeri
Scopus Q Değeri
Cilt
38
Sayı
2
