Development of molecular imprinting-based smart cryogels for selective recognition and separation of serum cytochrome-c as a biochemical indicator

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dc.contributor.authorCanpolat, Gurbet
dc.contributor.authorDolak, Ibrahim
dc.contributor.authorOnat, Ruken
dc.contributor.authorKecili, Rustem
dc.contributor.authorBaysal, Zubeyde
dc.contributor.authorZiyadanogullari, Berrin
dc.contributor.authorErsoz, Arzu
dc.date.accessioned2024-12-24T19:27:33Z
dc.date.available2024-12-24T19:27:33Z
dc.date.issued2021
dc.departmentSiirt Üniversitesi
dc.description.abstractThe recognition and detection of proteins has an important role in the fields of biochemistry and medicine in term of diagnosis and prognosis. In this study, molecularly imprinted cryogel (MIP) was synthesized for the selective recognition of Cytochrome-c (Cyt-c) by cryopolymerization techniques with the lanthanide-chelate approach using (MAAP)2-Ce(III) as complex functional monomer and Cyt-c as a template protein. The incorporation of template with monomer was characterized by near- infrared (Near-IR) spectroscopy. The binding capacity was optimized in accordance with numerous experimental parameters including pH, initial Cyt-c concentration, flow rate, temperature and ionic strength. As a result, the maximum binding capacity reached to 98.33 mg g-1 in a buffer system with a pH of 6.0. Beside of the excellent reusability performance of prepared MIP, it was successfully applied to separation of template Cyt-c in the existence of hemoglobin (Hb) and myoglobin (Mb) chosen as interfering proteins. The relative selectivity constants of the Cyt-imprinted polymer were determined as 13.84 for the Cyt/Mb pair and 16.05 for the Cyt/Hb pair. Consequently, a high selectivity, simple and cheap strategy for efficient separation of proteins was provided with this novel MIP cryogel.
dc.description.sponsorshipScientific Research Project Coordinator Unit of Dicle University [FEN.17.001]
dc.description.sponsorshipThis study was financially supported by the Scientific Research Project Coordinator Unit of Dicle University (Project No. FEN.17.001) . The authors would like to thanks the Scientific Research Project Coordinator of Dicle University.
dc.identifier.doi10.1016/j.procbio.2021.04.005
dc.identifier.endpage119
dc.identifier.issn1359-5113
dc.identifier.issn1873-3298
dc.identifier.scopus2-s2.0-85105691691
dc.identifier.scopusqualityQ1
dc.identifier.startpage112
dc.identifier.urihttps://doi.org/10.1016/j.procbio.2021.04.005
dc.identifier.urihttps://hdl.handle.net/20.500.12604/6700
dc.identifier.volume106
dc.identifier.wosWOS:000655324500002
dc.identifier.wosqualityQ2
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.language.isoen
dc.publisherElsevier Sci Ltd
dc.relation.ispartofProcess Biochemistry
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.snmzKA_20241222
dc.subjectBiomarker
dc.subjectCytochrome-c
dc.subjectLanthanide chelate
dc.subjectMolecularly imprinted polymer
dc.subjectSelective recognition
dc.titleDevelopment of molecular imprinting-based smart cryogels for selective recognition and separation of serum cytochrome-c as a biochemical indicator
dc.typeArticle

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