A Molecular survey of Hepatozoon canis in dogs in the Siirt province of Turkey

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dc.contributor.authorCelik, Burcak Aslan
dc.contributor.authorCelik, Ozgur Yasar
dc.contributor.authorAyan, Adnan
dc.contributor.authorYilmaz, Ali Bilgin
dc.contributor.authorKilinc, Ozlem Orunc
dc.contributor.authorAyan, Ozge Oktay
dc.date.accessioned2024-12-24T19:30:50Z
dc.date.available2024-12-24T19:30:50Z
dc.date.issued2022
dc.departmentSiirt Üniversitesi
dc.description.abstractThis study aimed to determine Hepatozoon canis prevalence in dogs in the Siirt province of Turkey by the molecular method. The animal material of the study consisted of a total of 75 dogs that appeared clinically healthy. Two ml of blood sample were taken from the vena cephalica antebrachii. Then, DNA extraction was performed. Polymerase chain reaction (PCR) was performed to amplify the 666 bp 18S rRNA gene region of Hepatozoon canis. Two positive PCR products were purified and sequenced. As a result of Nested-PCR, H. canis specific bands in 666 bp size were obtained in 7 (9.33%) out of 75 dogs. The result of sequence analysis, the nucleotide sequence was registered in the NCBI GenBank database with accession numbers OL467380.1-OL467538.1. Hepatozoon canis registered in GenBank of sequence OL467380.1 was found to be similar with other H. canis strains of registration numbers MW684292.1 with 99.69% and MH615006.1-MK091085.1-MF797806.1 with 99.53% rates; and the sequence with registration number OL467538.1 was found to be similar to the series MW684291.1 with 99.09% and MH615006.1-MK091085.1-KX 818220.1 with 99.08% rates by BLAST analysis. Hepatozoon canis prevalence of dogs in the Siirt province was determined as a result of this study. It is of great importance to take preventive measures, especially to fight ticks with appropriate acaricides, since there is no vaccine to prevent the disease.
dc.identifier.doi10.2754/avb202291030277
dc.identifier.endpage283
dc.identifier.issn0001-7213
dc.identifier.issn1801-7576
dc.identifier.issue3
dc.identifier.scopus2-s2.0-85134494506
dc.identifier.scopusqualityQ3
dc.identifier.startpage277
dc.identifier.urihttps://doi.org/10.2754/avb202291030277
dc.identifier.urihttps://hdl.handle.net/20.500.12604/7702
dc.identifier.volume91
dc.identifier.wosWOS:000834835300008
dc.identifier.wosqualityQ4
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.language.isoen
dc.publisherVeterinarni A Farmaceuticka Univerzita Brno
dc.relation.ispartofActa Veterinaria Brno
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.snmzKA_20241222
dc.subjectHepatozoon canis
dc.subjectPCR
dc.subjectDNA extraction
dc.subjectsequence
dc.titleA Molecular survey of Hepatozoon canis in dogs in the Siirt province of Turkey
dc.typeArticle

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