Texture-Based Segmentation and a New Cell Shape Index for Quantitative Analysis of Cell Spreading in AFM Images

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dc.contributor.authorTiryaki, Volkan
dc.contributor.authorAdia-Nimuwa, Usienemnfon
dc.contributor.authorAyres, Virginia M
dc.contributor.authorAhmed, Ijaz
dc.contributor.authorShreiber, David I
dc.date.accessioned2017-05-04T08:54:13Z
dc.date.available2017-05-04T08:54:13Z
dc.date.issued2015-11-02
dc.departmentBelirleneceken_US
dc.description.abstractA new cell shape index is defined for use with atomic force microscopy height images of cell cultures. The new cell shape index reveals quantitative cell spreading information not included in a conventional cell shape index. A supervised learning-based cell segmentation algorithm was implemented by texture feature extraction and a multi-layer neural network classifier. The texture feature sets for four different culture surfaces were determined from the gray level co-occurrence matrix and local statistics texture models using two feature selection algorithms and by considering computational cost. The quantitative morphometry of quiescent-like and reactive-like cerebral cortical astrocytes cultured on four different culture environments was investigated using the new and conventional cell shape index. Inclusion of cell spreading with stellation information through use of the new cell shape index was shown to change biomedical conclusions derived from conventional cell shape analysis based on stellation alone. The new CSI results showed that the quantitative astrocyte spreading and stellation behavior was induced by both the underlying substrate and the immunoreactivity of the astrocytesen_US
dc.description.sponsorshipNational Science Foundation, Grant numbers: PHY-0957776; ARRACBET-0846328; The General Directorate of High Education, Ministry of National Education of the Republic of Turkey.en_US
dc.identifier.citationTiryaki, VM, Adia-Nimuwa, U, Ayres, VM, Ahmed, I, Shreiber, DI. Texture-based segmentation and a new cell shape index for quantitative analysis of cell spreading in AFM images. Cytometry: Part A, 2015, 87(12): 1090-1100 (DOI: 10.1002/cyto.a.22739).en_US
dc.identifier.otherDOI: 10.1002/cyto.a.22739
dc.identifier.pmid26524224
dc.identifier.urihttps://hdl.handle.net/20.500.12604/457
dc.identifier.wosWOS:000367670800005
dc.identifier.wosqualityQ2
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakPubMed
dc.language.isoenen_US
dc.publisherCytometry Part Aen_US
dc.relation.publicationcategoryUluslararası Hakemli Dergi Makalesien_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.snmzKG_20241224
dc.subjectcell spreading; stellation; immunoreactivity; texture-based cell segmentation; floating feature selection; cell–material interactionsen_US
dc.titleTexture-Based Segmentation and a New Cell Shape Index for Quantitative Analysis of Cell Spreading in AFM Imagesen_US
dc.typeArticleen_US

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