The evaluation of the protective effect of ambroxol against acetaminophen-induced hepatorenal toxicity in rats

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dc.contributor.authorSayiner, Serkan
dc.contributor.authorGencosman, Sevgi
dc.contributor.authorSah, Huseyin
dc.contributor.authorGulmez, Nurhayat
dc.contributor.authorSehirli, Ahmet Ozer
dc.date.accessioned2024-12-24T19:30:51Z
dc.date.available2024-12-24T19:30:51Z
dc.date.issued2024
dc.departmentSiirt Üniversitesi
dc.description.abstractAcetaminophen (APAP), widely used as an analgesic-antipyretic drug, can cause liver and kidney damage at high doses. This study explored the protective effects of a mucolytic agent and an antioxidant Ambroxol (AMB), against APAP-induced toxicity in rats. The experiment included four groups of Wistar albino rats each having 6 animals in both sexes: a control group, an AMB-only group (50 mg/kg orally), an APAP-only group (1000 mg/kg intraperitoneally), and a combination APAP+AMB group. Twenty-four hours following the administration of APAP administration, rats were sacrificed. Measurements of blood levels of liver enzymes (AST, ALT, ALP, LDH), kidney function markers (Urea, Creatinine), and antioxidant enzymes (SOD, GPx) were performed. GPx and SOD activities were also assessed in hepatic and renal tissue samples. Histological examination of hepatic and renal tissues was conducted using Haematoxylin and Eosin staining. Results showed that APAP significantly increased liver enzymes, BUN, and Creatinine levels, indicating hepatorenal damage. This was accompanied by a decrease in plasma GPx and SOD activities. However, AMB treatment significantly mitigated these changes. It improved enzyme activities and increased hepatic GPx. Histologically, the APAP group showed liver cell damage, necrosis, haemorrhage, and inflammation, which were notably reduced in the AMB-treated group. This study suggests that Ambroxol effectively counters APAPinduced hepatorenal damage by restoring antioxidant enzyme levels and normalizing functional enzyme activities, highlighting its potential as a protective agent.
dc.description.sponsorshipAcknowledgements: This research was partially presented in an oral format at the 2nd International Conference on Veterinary Biochemistry & Clinical Biochemistry, held from October 24 to 26, 2019, in Ankara, Turkey.
dc.identifier.doi10.29228/jrp.802
dc.identifier.endpage1219
dc.identifier.issn2630-6344
dc.identifier.issue4
dc.identifier.scopus2-s2.0-85202468339
dc.identifier.scopusqualityQ3
dc.identifier.startpage1210
dc.identifier.urihttps://doi.org/10.29228/jrp.802
dc.identifier.urihttps://hdl.handle.net/20.500.12604/7714
dc.identifier.volume28
dc.identifier.wosWOS:001288934400028
dc.identifier.wosqualityN/A
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.language.isoen
dc.publisherMarmara Univ
dc.relation.ispartofJournal of Research in Pharmacy
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.snmzKA_20241222
dc.subjectAcetaminophen
dc.subjectAmbroxol
dc.subjectGlutathione peroxidase
dc.subjectSuperoxide dismutase
dc.subjectLiver
dc.subjectKidney
dc.titleThe evaluation of the protective effect of ambroxol against acetaminophen-induced hepatorenal toxicity in rats
dc.typeArticle

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