The effect of UV-C on the total antioxidant activity and phenolic content in hypericum scabroides raised under in vitro conditions

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dc.authorid0000-0003-3960-9553en_US
dc.contributor.authorNamlı, Sureyya, Akcan, Nurullah
dc.date.accessioned2019-11-28T21:27:12Z
dc.date.available2019-11-28T21:27:12Z
dc.date.issued2014en_US
dc.departmentSiirt Sağlık Yüksekokuluen_US
dc.description.abstractSeeds of H. scabroides Robson and Poulter were germinated in vitro in Murashige and Skoog (MS) medium without hormones. The shoots thus obtained were then reproduced successfully in MS medium supplemented with 0.25 mg L-1 BAP (N6-benzylaminopurine). The shoots, into two groups of different periods of time (15, 30, 45, 60 min.) were exposed to UV-C as direct (covers of culture vessels are open) and indirect (covers of culture vessels are closed). After treatment, the antioxidant activities of the extracts obtained from H. scabroides were determined using the DPPH test method. The samples had lower activities than ascorbic acid (AA), butylated hydroxyanisole (BHA) and butylated hydroxytoluene (BHT). In this work, extracts exposed for 15 min to direct UV-C had higher antioxidant activity (84.5 ± 0.4 I%) than extracts exposed for 30, 45, 60 min or control extracts (80.6 ± 0.7 I%). The results indicated that the control group extracts had a higher antioxidant activity than extracts exposed to 30, 45, 60 min or 15, 30, 45, 60 min of direct and indirect UV-C respectively. In the current study, brief UV-C exposure led to an increase in the amount of antioxidant activity of H. scabroides extracts, particularly for direct and indirect 15-min doses of UV-C. However, only the extracts exposed to15 min of direct UV-C had a higher response with respect to total phenolic content (837±2.1 μg/mg) than the control group (815±1.3 μg/mg).en_US
dc.description.provenanceSubmitted by NURULLAH AKCAN (nakcan@siirt.edu.tr) on 2019-11-28T21:27:12Z No. of bitstreams: 1 Akcan Nurullah-süreyya.pdf: 492773 bytes, checksum: 07a33c0d0c635266e19db297ad954c5e (MD5)en
dc.description.provenanceMade available in DSpace on 2019-11-28T21:27:12Z (GMT). No. of bitstreams: 1 Akcan Nurullah-süreyya.pdf: 492773 bytes, checksum: 07a33c0d0c635266e19db297ad954c5e (MD5) Previous issue date: 2014en
dc.identifier.endpage18en_US
dc.identifier.issue3en_US
dc.identifier.scopus2-s2.0-84894672364
dc.identifier.scopusqualityQ4
dc.identifier.startpage12en_US
dc.identifier.urihttps://hdl.handle.net/20.500.12604/2052
dc.identifier.volume9en_US
dc.identifier.wosWOS:000334936900002
dc.identifier.wosqualityQ4
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.institutionauthorAkcan, Nurullah
dc.language.isoenen_US
dc.relation.ispartofResearch Journal of Biotechnology
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.snmzKG_20241224
dc.subjectHypericum scabroides, in vitro, UV-C radiation, antioxidant, total phenolicen_US
dc.titleThe effect of UV-C on the total antioxidant activity and phenolic content in hypericum scabroides raised under in vitro conditionsen_US
dc.typeArticleen_US

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