Heavy metal ion inhibition studies of human, sheep and fish ?-carbonic anhydrases
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Tarih
2013
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Dergi ISSN
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Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Carbonic anhydrases (CAs, EC 4.2.1.1) were purified from sheep kidney (sCA IV), from the liver of the teleost fish Dicentrarchus labrax (dCA) and from human erythrocytes (hCA I and hCA II). The purification procedure consisted of a single step affinity chromatography on Sepharose 4B-tyrosine-sulfanilamide. The kinetic parameters of these enzymes were determined for their esterase activity with 4-nitrophenyl acetate as substrate. The following metal ions, Pb2+, Co2+, Hg2+, Cd2+, Zn2+, Se2+, Cu2+, Al3+ and Mn3+ showed inhibitory effects on these enzymes. The tested metal ions inhibited these CAs competitively in the low milimolar/submillimolar range. The susceptibility to various cations inhibitors differs significantly between these vertebrate ?-CAs and is probably due to their binding to His64 or the histidine cluster.
Açıklama
Anahtar Kelimeler
Carbonic anhydrase, metal ion, erythrocyte, sheep kidney, teleost fish
