Molecularly imprinted polymer-based microspheres for selective extraction of hemoglobin from blood serum

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dc.contributor.authorCanpolat, Gurbet
dc.date.accessioned2024-12-24T19:27:33Z
dc.date.available2024-12-24T19:27:33Z
dc.date.issued2023
dc.departmentSiirt Üniversitesi
dc.description.abstractIn this study, a highly selective molecularly imprinted microsphere (Hb-MAFol-Nd(III)-MIP) was prepared with ion-chelate approach for the extraction of hemoglobin (Hb) from blood serum. The Hb-imprinted microspheres were synthesized by suspension polymerization. For this purpose, Hb, N-methacryloylamino folic acid-Nd(III) (MAFol-Nd(III)), ethtylene glycol dimethacrylate (EDMA) and azobisisobutyronitrile (AIBN) were used as the template, complex functional monomer, cross-linker and initiator, respectively. Imprinted and NIP microspheres were characterized by Fourier Transform Infrared Spectroscopy (FT-IR) and Scanning Electron Microscopy (SEM). The binding efficiency of the template on both MIP and NIP was optimized with respect to various parameters including pH, contact time, and hemoglobin concentration. As a result, the maximum binding capacity was observed to be as 361 mg g(-1) at the pH value 7.0. For selectivity study, myoglobin (Mb), cytochrome c (Cyt) and lysozyme (Lys) were chosen as competitive proteins. The prepared imprinted microspheres exhibited high selectivity toward template based on the selectivity coefficients (k). The extraction of Hb from spiked blood serum sample was performed with excellent success. From the correlation coefficients, isotherm study indicated that the adsorption of Hb by MIP fit a Langmuir model.
dc.description.sponsorshipSiirt University Scientific Research Unit (BAP) [2018-SI??, FED-038]
dc.description.sponsorshipAcknowledgements This work was supported by Siirt University Scientific Research Unit (BAP) [2018-SI??FED-038] . The author is grateful to Dr. I?brahim DOLAK for allowing to use his synthesized monomer MAFol with regardless of conflict of interest.
dc.identifier.doi10.1016/j.procbio.2023.03.017
dc.identifier.endpage93
dc.identifier.issn1359-5113
dc.identifier.issn1873-3298
dc.identifier.scopus2-s2.0-85150056593
dc.identifier.scopusqualityQ1
dc.identifier.startpage86
dc.identifier.urihttps://doi.org/10.1016/j.procbio.2023.03.017
dc.identifier.urihttps://hdl.handle.net/20.500.12604/6701
dc.identifier.volume129
dc.identifier.wosWOS:000952640800001
dc.identifier.wosqualityQ2
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.language.isoen
dc.publisherElsevier Sci Ltd
dc.relation.ispartofProcess Biochemistry
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.snmzKA_20241222
dc.subjectHemoglobin
dc.subjectFolic acid
dc.subjectNeodymium(III)
dc.subjectMolecularly imprinted polymer
dc.titleMolecularly imprinted polymer-based microspheres for selective extraction of hemoglobin from blood serum
dc.typeArticle

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