Genetic Relatedness in Elite Cultivars of Moth Bean using Morpho-agronomic and Molecular Markers

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dc.contributor.authorChoudhary, K. B.
dc.contributor.authorSharma, R.
dc.contributor.authorSolanki, R. K.
dc.contributor.authorMahla, H. R.
dc.contributor.authorJadon, K. S.
dc.contributor.authorChoudhary, Mukesh
dc.contributor.authorWani, Shabir H.
dc.date.accessioned2024-12-24T19:30:33Z
dc.date.available2024-12-24T19:30:33Z
dc.date.issued2024
dc.departmentSiirt Üniversitesi
dc.description.abstractBackground: Moth bean is a very nutritive and hardy crop, especially for resource poor -arid agriculture. The limited use of cultivars in moth bean breeding resulted into narrow genetic base. This study aimed to diversify the conical genetic base and developimproved cultivars, through molecular (RAPD) and morpho-agronomic characterization of commercially elite moth bean cultivars. Methods: Elite moth bean cultivars (15) were evaluated for 11 morpho-agronomic traits. Eleven RAPD markers were used to amplify genomic DNA and perform molecular characterization. Clustering approach was used for grouping of the cultivars. Result: RAPD markers revealed high polymorphism in 15 elite moth bean cultivars. The polymorphism information content (PIC) values varied within the range of 0.45 to 0.88, with an average of 0.77. The number of alleles at different loci ranged from 4 to 16, with an average of 10 alleles per locus. When employing UPGMA-based cluster analysis, utilizing 11 morpho-agronomic traits, the cultivars were grouped into four clusters. However, when RAPD markers were used, the cultivars were grouped into five clusters. The Jaccard's similarity coefficient and Manhattan dissimilarity coefficient fell within the ranges of 0.18 to 0.70 and 0.02 to 0.42, respectively. These values signify the degree of genetic variability within the cultivars. Furthermore, a Mantel test was conducted to examine the correlation between agronomic traits and the RAPD-based matrix. The results showed a negative correlation, but it was not statistically significant.The high PIC values and the successful amplification of multiple loci demonstrate the efficacy of RAPD markers in assessing genetic diversity in moth beans. The study revealed enormous genetic variation among cultivars and crosses can be attempted between cultivars of different groups to create better recombinants in moth bean breeding programmes.
dc.description.sponsorshipKing Saud University, Riyadh, Saudi Arabia [RSP2024R298]
dc.description.sponsorshipThe authors extend their appreciation to the Researchers Supporting Project number (RSP2024R298) , King Saud University, Riyadh, Saudi Arabia.
dc.identifier.doi10.18805/LRF-774
dc.identifier.endpage384
dc.identifier.issn0250-5371
dc.identifier.issue3
dc.identifier.scopus2-s2.0-85189618360
dc.identifier.scopusqualityQ3
dc.identifier.startpage378
dc.identifier.urihttps://doi.org/10.18805/LRF-774
dc.identifier.urihttps://hdl.handle.net/20.500.12604/7583
dc.identifier.volume47
dc.identifier.wosWOS:001260909700006
dc.identifier.wosqualityN/A
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.language.isoen
dc.publisherAgricultural Research Communication Centre
dc.relation.ispartofLegume Research
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.snmzKA_20241222
dc.subjectCataloguing
dc.subjectCluster analysis
dc.subjectDiversity
dc.subjectMoth bean
dc.subjectRAPD.
dc.titleGenetic Relatedness in Elite Cultivars of Moth Bean using Morpho-agronomic and Molecular Markers
dc.typeArticle

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