Indirect organogenesis and in vitro bulb formation of Pancratium maritimum

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dc.authoridBUYUKALACA, ORHAN/0000-0002-0402-7214
dc.authoridyasemin, sara/0000-0003-2193-6791
dc.contributor.authorYasemin, Sara
dc.contributor.authorKoksal, Nezihe
dc.contributor.authorBuyukalaca, Saadet
dc.date.accessioned2024-12-24T19:24:50Z
dc.date.available2024-12-24T19:24:50Z
dc.date.issued2023
dc.departmentSiirt Üniversitesi
dc.description.abstractSea daffodil (Pancratium maritimum L.), which belongs to the Amaryllidaceae family, grows in sandy areas along coastal regions and its population is at risk due to the exploitation of its natural resources for medicinal purposes, tourism, and urbanization. Micropropagation is employed to conserve and sustain endangered plant species like P. maritimum. In this investigation, different types of explants (leaf, root, and mature zygotic embryos) from P. maritimum were cultured on Murashige-Skoog (MS) medium supplemented with 2,4-Dichlorophenoxyacetic acid (2,4-D) (1, 2, 4 mg l(-1)) and 6-Benzyladenine (BA) (0 and 1 mg l(-1)) for callus induction. The callus formation rate, callus growth rate, embryogenic callus rate, and callus type were evaluated. The induced calli were further tested for shoot formation on MS medium supplemented with 2 mg l(-1) BA and 0.2 and 0.5 mg l(-1) 2,4-D. The results showed that the highest callus induction was achieved using zygotic embryo explants and the medium containing both 2,4-D and BA. Successful shoot formation from callus was determined using both MS media supplemented with 2 mg l(-1) BA and 0.5 or 0.2 mg l(-1) 2,4-D, with a success rate of 90%. To induce in vitro bulb formation of P. maritimum plantlets, MS medium containing varying concentrations of sucrose (20, 40, and 80 g l(-1)), BA (0, 1, and 2 mg l(-1)), and 2,4-D (0, 0.1, and 0.2 mg l(-1)) was utilized. The bulb formation rate of P. maritimum was successful in all growth media, ranging from 60 to 82%. The diameter of the bulb was found to increase with higher sucrose concentration (80 g l(-1)) in the growth medium. The impact of plant growth regulators on bulb weight was more pronounced in nutrient medium containing low sucrose concentration (20 g l(-1)). Efficient protocols for embryogenic callus induction, organogenesis, regeneration, bulb formation, and acclimatization were developed for P. maritimum, providing valuable insights for future studies. Key messagePancratium maritimum is an endangered plant with medicinal and decorative value. We have successfully developed a comprehensive protocol for its micropropagation, including callus induction, regeneration, bulb formation, and acclimatization.
dc.description.sponsorship[FBA-2015-4083]
dc.description.sponsorshipAcknowledgementsData presented in the study were obtained from the MSc thesis of Sara Yasemin (corresponding author). Special thanks are due to the Cukurova University, Scientific Research Projects Coordinating Office (Project No: FBA-2015-4083) for supporting the present study.
dc.identifier.doi10.1007/s11240-023-02545-z
dc.identifier.endpage727
dc.identifier.issn0167-6857
dc.identifier.issn1573-5044
dc.identifier.issue3
dc.identifier.scopus2-s2.0-85162068919
dc.identifier.scopusqualityQ1
dc.identifier.startpage713
dc.identifier.urihttps://doi.org/10.1007/s11240-023-02545-z
dc.identifier.urihttps://hdl.handle.net/20.500.12604/6131
dc.identifier.volume154
dc.identifier.wosWOS:001006900500001
dc.identifier.wosqualityQ2
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.language.isoen
dc.publisherSpringer
dc.relation.ispartofPlant Cell Tissue and Organ Culture
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.snmzKA_20241222
dc.subjectBulb formation
dc.subjectCallus induction
dc.subjectMicropropagation
dc.subjectPlant growth regulators
dc.subjectRegeneration
dc.subjectSea daffodil
dc.titleIndirect organogenesis and in vitro bulb formation of Pancratium maritimum
dc.typeArticle

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