Production andOptimization Parameters of Amylases from Bacillus subtilis RSKK96Under Solid State Fermentation
Yükleniyor...
Dosyalar
Tarih
2012
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Chemical and Biochemical Engineering Quarterly
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
The production of extracellular -amylase by producing Bacillus subtilis RSKK96
was studied under solid state fermentation (SSF). Various agroresidues as substrate were
studied for enzyme production. The highest enzyme yield was expressed with cotton
stalk substrate (1016.3 ± 32.6 U g–1 · 10–3). Production parameters were optimized
as incubation time 72 hours, incubation temperature 37 °C, agitation speed 150 rpm,
inoculum size 35 %, initial moisture content 30 %, initial pH 7.0. Supplementation of the
fermentation medium with carbon and metal salt sources decreased the enzyme production.
Among different nitrogen sources supplemented, ammonium nitrate (1 %) showed
maximum enzyme production (1483.1 ± 32.5 U g–1 · 10–3). Enzyme activity was observed
as 73 % at 100 °C.
Açıklama
Anahtar Kelimeler
Amylase, Bacillus subtilis RSKK96, solid substrate fermentation, cotton stalk
Kaynak
Chemical and Biochemical Engineering Quarterly
WoS Q Değeri
Scopus Q Değeri
Cilt
26
Sayı
3
