Texture-based segmentation and a new cell shape index for quantitative analysis of cell spreading in AFM images
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Tarih
2015
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Wiley-Liss Inc.
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
A new cell shape index is defined for use with atomic force microscopy height images of cell cultures. The new cell shape index reveals quantitative cell spreading information not included in a conventional cell shape index. A supervised learning-based cell segmentation algorithm was implemented by texture feature extraction and a multi-layer neural network classifier. The texture feature sets for four different culture surfaces were determined from the gray level co-occurrence matrix and local statistics texture models using two feature selection algorithms and by considering computational cost. The quantitative morphometry of quiescent-like and reactive-like cerebral cortical astrocytes cultured on four different culture environments was investigated using the new and conventional cell shape index. Inclusion of cell spreading with stellation information through use of the new cell shape index was shown to change biomedical conclusions derived from conventional cell shape analysis based on stellation alone. The new CSI results showed that the quantitative astrocyte spreading and stellation behavior was induced by both the underlying substrate and the immunoreactivity of the astrocytes. © 2015 International Society for Advancement of Cytometry.
Açıklama
Anahtar Kelimeler
Cell spreading, Cell-material interactions, Floating feature selection, Immunoreactivity, Stellation, Texture-based cell segmentation
Kaynak
Cytometry Part A
WoS Q Değeri
Scopus Q Değeri
Q1
Cilt
87
Sayı
12
