In vitro propagation of Diyarbakir watermelons and comparison of direct-seeded and transplanted watermelon

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dc.authoridOkumus, Veysi/0000-0002-5505-2700
dc.contributor.authorOkumus, Veysi
dc.contributor.authorPirinc, Vedat
dc.contributor.authorOnay, Ahmet
dc.contributor.authorBasaran, Davut
dc.date.accessioned2024-12-24T19:33:57Z
dc.date.available2024-12-24T19:33:57Z
dc.date.issued2011
dc.departmentSiirt Üniversitesi
dc.description.abstractA rapid protocol using shoot tip explants for micropropagation of Diyarbakir watermelon [Citrullus lanatus (Thunb.) Matsum. & Nakai] types Surme, Beyazkis and Karakis was achieved. Shoot tips from 5-day-old in vitro germinated seedlings were cultured on shoot regeneration medium for 3 weeks. The effects of the different concentrations of benzyl adenine (BA) and carbohydrate types on shoot proliferation were examined. The results confirmed that a range of 0.5-1.0 mg/L of BA was almost equally effective in promoting the shoot length of cultures in the 3 genotypes. Sucrose, in the presence of BA, was superior to other carbohydrates for the 3 genotypes studied in terms of the number of proliferated shoots and the average shoot length obtained. The highest percentage of rooting was achieved when medium supplemented with 1.0 mg/L of indole-3-butyric acid (IBA) was used for the 3 genotypes. The highest frequency of acclimatized plantlets for the 3 genotypes was 85%, 85%, and 90%, respectively, in sterile compost, when the shoots of Beyazkis, Karakis, and Surme were rooted in the IBA-supplemented rooting treatments. The Surme genotype produced a significantly higher yield and mean fruit weight than the Beyazkis and Karakis genotypes in both direct-seeded and transplanted watermelons. The main soluble sugar of the experimental types was separated, identified, and quantified using high performance liquid chromatography (H PLC). Fructose was found to be the most abundant sugar and was highly detected in Beyazkis for the seeded watermelon and in Karakis for the transplanted watermelon. This demonstrates that in vitro propagation can be used to produce high quality diploid Diyarbakir watermelon for use in breeding lines.
dc.description.sponsorshipDUAPK, the Dicle University Research Project Council [DUBAP 07-02-14]
dc.description.sponsorshipThis study was funded by a grant from DUAPK, the Dicle University Research Project Council (DUBAP 07-02-14). The authors thank Dr. Ersin Kilinc for HPLC quantitive data of sugar.
dc.identifier.doi10.3906/biy-1001-11
dc.identifier.endpage610
dc.identifier.issn1300-0152
dc.identifier.issn1303-6092
dc.identifier.issue5
dc.identifier.scopus2-s2.0-80052682945
dc.identifier.scopusqualityQ1
dc.identifier.startpage601
dc.identifier.urihttps://doi.org/10.3906/biy-1001-11
dc.identifier.urihttps://hdl.handle.net/20.500.12604/8343
dc.identifier.volume35
dc.identifier.wosWOS:000295771900009
dc.identifier.wosqualityQ3
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.language.isoen
dc.publisherTubitak Scientific & Technological Research Council Turkey
dc.relation.ispartofTurkish Journal of Biology
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.snmzKA_20241222
dc.subjectWatermelon
dc.subjectregeneration
dc.subjecttissue culture
dc.subjectyield
dc.subjectfruit
dc.titleIn vitro propagation of Diyarbakir watermelons and comparison of direct-seeded and transplanted watermelon
dc.title.alternativeDiyarbakır karpuzunun in vitro ço?altılması ve do?rudan tohumdan ve aktarılmış fidelerden elde edilen karpuzların karşılaştırılması
dc.typeArticle

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