Investigation of selected bacterial agents causing sheep abortion in the Van Province by RT-PCR and histopathological methods

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dc.authoridGULAYDIN, Ozgul/0000-0001-8376-2008
dc.authoridIlhan, Fatma/0000-0003-0363-6285
dc.authoridozturk, cihat/0000-0003-2868-2317
dc.authoridEKIN, Ismail Hakki/0000-0001-5029-8130
dc.contributor.authorGulaydin, Ozgul
dc.contributor.authorOzturk, Cihat
dc.contributor.authorEkin, Ismail Hakki
dc.contributor.authorIlhan, Ziya
dc.contributor.authorIlhan, Fatma
dc.date.accessioned2024-12-24T19:30:50Z
dc.date.available2024-12-24T19:30:50Z
dc.date.issued2023
dc.departmentSiirt Üniversitesi
dc.description.abstractAbortion causes significant economic losses in the sheep industry. Determination of the aetiology is important in dealing with abortions. The present study was aimed to identify selected important bacterial pathogens in the abortion cases of sheep. A total of 113 samples (105 aborted sheep foetuses, 4 placentas, and 4 vaginal swab samples) from 85 different sheep flocks were examined by real-time PCR (RT-PCR) regarding Chlamydia (C.) spp., C. abortus, Brucella (B.) spp., B. melitensis, Salmonella (S.) spp., S. Abortusovis, Coxiella (C.) burnetii, Listeria (L.) spp., L. monocytogenes, and Campylobacter spp. All cases that were found to be positive for bacterial agents by RT-PCR, were examined pathologically. Tissue samples of foetuses that were found to be positive for B. melitensis and L. monocytogenes by RT-PCR were also investigated immunohistochemically. A total of 35 (30.9%) samples were found to be positive by RT-PCR, with 15 (42.8%), 9 (25.7%), 5 (14.2%), 4 (11.4%), 1 (2.8%), and 1 (2.8%) of them being identified as C. abortus, B. melitensis, S. Abortusovis, C. burnetii, L. monocytogenes and Campylobacter spp., respectively. The presence of the antigen was confirmed also immunohistochemically in the cases with B. melitensis and L. monocytogenes. As a consequence, C. abortus was found to cause the highest rate of sheep abortion cases, which should be taken into account when implementing control measures in epidemiological investigations.
dc.description.sponsorshipScientific Research Projections of Van Yuezuencue Yil University [TSA-2017-5841]
dc.description.sponsorshipThis study is part of the project funded by Scientific Research Projections of Van Yuezuencue Yil University (TSA-2017-5841). A summary of this study was presented as an oral presentation on ISPEC 7th International Conference on Agriculture, Animal Sciences and Rural Development.
dc.identifier.doi10.2754/avb202392010069
dc.identifier.endpage+
dc.identifier.issn0001-7213
dc.identifier.issn1801-7576
dc.identifier.issue1
dc.identifier.scopus2-s2.0-85149232087
dc.identifier.scopusqualityQ3
dc.identifier.startpage69
dc.identifier.urihttps://doi.org/10.2754/avb202392010069
dc.identifier.urihttps://hdl.handle.net/20.500.12604/7703
dc.identifier.volume92
dc.identifier.wosWOS:000933978400010
dc.identifier.wosqualityQ3
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.language.isoen
dc.publisherVeterinarni A Farmaceuticka Univerzita Brno
dc.relation.ispartofActa Veterinaria Brno
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.snmzKA_20241222
dc.subjectOvine
dc.subjectruminants
dc.subjectreproduction
dc.subjectimmunohistochemistry
dc.titleInvestigation of selected bacterial agents causing sheep abortion in the Van Province by RT-PCR and histopathological methods
dc.typeArticle

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