INHIBITORY EFFECTS OF SOME PESTICIDES AND METALS ON CARBONIC ANHYDRASE PURIFIED FROM SHABUT FISH (BARBUS GRYPUS) GILL TISSUE

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dc.contributor.authorYerlikaya, E.
dc.date.accessioned2024-12-24T19:30:15Z
dc.date.available2024-12-24T19:30:15Z
dc.date.issued2019
dc.departmentSiirt Üniversitesi
dc.description.abstractCarbonic anhydrase enzyme catalyzes the reversible inter-conversion of CO2 and HCO3. The enzyme is crucial for the osmotic balance and acid base regulation in the fish. It is well-known that gills of fish play the most important role in acid base relevant ion transfer, the transfer of H+ and/or HCO3, for the maintenance of systemic pH. Many researches have shown that fish are the species that is the most susceptible to environmental toxins. In addition, these toxins firstly encounter the gill tissue in fish. In this study, the carbonic anhydrase enzyme was purified 198.6-folds with 58.8% yield from Shabut Fish (Barbus grypus) gill tissue by Sepharose-4B-L-tyrosine-sulfanilamide affinity column. The specific activity was determined as 2.92 EU/mg protein. The molecular weight determined by sodium dodecyl sulfate polyacrylamide gel electrophoresis was found to be about 29.9 kDa. Inhibitory effects of some pesticides (Spinosad and Dimethoate) and metal ions (Al3+, Cu2+, Ba2+, Fe2+, M2+, Se2+) were examined on the purified carbonic anhydrase enzyme. Inhibition graphics were drawn in order to find the IC50 values of metals and pesticides showing inhibition. The kinetic parameters of this enzyme were determined for its esterase activity, with 4-nitrophenyl acetate as substrate.
dc.identifier.doi10.15666/aeer/1706_1347313483
dc.identifier.endpage13483
dc.identifier.issn1589-1623
dc.identifier.issn1785-0037
dc.identifier.issue6
dc.identifier.scopus2-s2.0-85077382464
dc.identifier.scopusqualityQ3
dc.identifier.startpage13473
dc.identifier.urihttps://doi.org/10.15666/aeer/1706_1347313483
dc.identifier.urihttps://hdl.handle.net/20.500.12604/7443
dc.identifier.volume17
dc.identifier.wosWOS:000505251300057
dc.identifier.wosqualityQ4
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.language.isoen
dc.publisherCorvinus Univ Budapest
dc.relation.ispartofApplied Ecology and Environmental Research
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.snmzKA_20241222
dc.subjectpollution
dc.subjectinhibition
dc.subjectenzyme
dc.subjectchromatography
dc.subjectcatalysis
dc.titleINHIBITORY EFFECTS OF SOME PESTICIDES AND METALS ON CARBONIC ANHYDRASE PURIFIED FROM SHABUT FISH (BARBUS GRYPUS) GILL TISSUE
dc.typeArticle

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