BREAKING SEED DORMANCY AND MICROPROPAGATION OF PERENNIAL VULNERARIA MILKVETCH (Astragalus vulnerariae DC.)

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dc.authoridDilaver, Zuhal/0000-0002-4923-3740
dc.contributor.authorDilaver, Zuhal
dc.contributor.authorMirzapour, Mohsen
dc.contributor.authorKendir, Hayrettin
dc.date.accessioned2024-12-24T19:30:46Z
dc.date.available2024-12-24T19:30:46Z
dc.date.issued2017
dc.departmentSiirt Üniversitesi
dc.description.abstractIn this study, an efficient system to break seed dormancy and plant regeneration was established for perennial vulneraria milkvetch (Astragalus vulnerariae DC.). The seed coat dormancy could be easily released using 40% sulphuric acid treatment for 15 minutes, which made the seed coat permeable without damaging zygotic embryos. The tissue culture studies reported regeneration on 5 explants, which had variable effects on shoot regeneration, using MS medium containing variable concentrations of Kin-NAA and BAP-NAA. Maximum shoot regeneration of 86.67% with 4.47 shoots per explant was noted on MS medium containing 0.5 mg center dot l(-1) Kin -0.5 mg center dot l(-1) NAA on hypocotyl explants. No shoot regeneration was noted on cotyledon leaf and shoot nodes using MS medium containing any concentration of BAP-NAA. Maximum shoot regeneration of 50% with 3 shoots per explant was also observed on 1 mg center dot l(-1) BAP-0.5 mg center dot l(-1) NAA on epicotyl explants. MS medium containing Kin-NAA induced hypocotyl explant shoots had rooting percentage of 36.67% on 0.5 mg center dot l(-1) IBA, whereas, MS medium containing BAP-NAA induced hypocotyl shoots had rooting percentage of 46.67%. The in vitro cultured plants had an acclimatization rate of 78%. Aesthetically attractive, economical, easy to maintain and water efficient vulneraria milkvetch is currently not available in landscaping. The system of plant regeneration and adaptation suggested by this paper may help to dissipate it to a broad range of water scarce environments as a sustainable and inexpensive choice.
dc.identifier.doi10.24326/asphc.2017.4.9
dc.identifier.endpage88
dc.identifier.issn1644-0692
dc.identifier.issue4
dc.identifier.scopus2-s2.0-85030092748
dc.identifier.scopusqualityQ3
dc.identifier.startpage79
dc.identifier.urihttps://doi.org/10.24326/asphc.2017.4.9
dc.identifier.urihttps://hdl.handle.net/20.500.12604/7671
dc.identifier.volume16
dc.identifier.wosWOS:000416066500009
dc.identifier.wosqualityQ4
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.language.isoen
dc.publisherWydawnictwo Akad Rolniczej W Lublinie
dc.relation.ispartofActa Scientiarum Polonorum-Hortorum Cultus
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.snmzKA_20241222
dc.subjectAstragalus vulnerariae DC.
dc.subjectvulneraria milkvetch
dc.subjectseed dormancy
dc.subjectmicropropagation
dc.subjectin vitro
dc.titleBREAKING SEED DORMANCY AND MICROPROPAGATION OF PERENNIAL VULNERARIA MILKVETCH (Astragalus vulnerariae DC.)
dc.typeArticle

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