Purification and Characterization of Carbonic Anhydrase from Agrı Balık Lake Trout Gill (Salmo trutta labrax) and Effects of Sulfonamides on Enzyme Activity

Basit Öğe Kaydı
dc.contributor.authorDemirdag, Ramazan
dc.contributor.authorComakli, Veysel
dc.contributor.authorKuzu, Muslum
dc.contributor.authorYerlikaya, Emrah
dc.contributor.authorSenturk, Murat
dc.date.accessioned2019-11-18T10:22:54Z
dc.date.available2019-11-18T10:22:54Z
dc.date.issued2015
dc.departmentBelirleneceken_US
dc.description.abstractCarbonic anhydrase (CA) was purified from Agrı Balık Lake trout gill (fCA) by affinity chromatography on a sepharose 4B-tyrosine-sulfanilamide column. The fCA enzyme was purified with about a 303.9 purification factor, a specific activity 4130.4 EU (mg-protein)–1, and a yield of 79.3 by using sepharose- 4B-L tyrosine-sulfanilamide affinity gel chromatography. The molecular weight determined by sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS–PAGE) was found to be about 29.9 kDa. The kinetic parameters, KM and Vmax were determined for the 4-nitrophenyl acetate hydrolysis reaction. Some sulfonamides were tested as inhibitors against the purified CA enzymes. The Ki constants for mafenide (1), p-toluenesulfonamide (2), 2-bromo-benzene sulfonamide (3), 4-chlorobenzene sulfonamide (4), 4-amino-6- chloro-1–3 benzenedisulfonamide (5), sulfamethazine (6), sulfaguanidine (7), sulfadiazine (8), and acetozazolamide (9) were in the range of 7.5–108.75 µM.en_US
dc.description.sponsorshipAgri Ibrahim Cecen University Scientific Research Projects Unit (BAP). Contract Grant Number: BAP 2012/MYO-02.en_US
dc.identifier.urihttps://hdl.handle.net/20.500.12604/1612
dc.language.isoenen_US
dc.relation.publicationcategoryUluslararası Hakemli Dergi Makalesien_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.snmz#KayıtKontrol#
dc.subjectAgrı Balık Lake Trout; Carbonic Anhydrase;Sulfonamides; Inhibition; Purification; Characterizationen_US
dc.titlePurification and Characterization of Carbonic Anhydrase from Agrı Balık Lake Trout Gill (Salmo trutta labrax) and Effects of Sulfonamides on Enzyme Activityen_US
dc.typeArticleen_US

Dosyalar

Lisans paketi
Listeleniyor 1 - 1 / 1
[ X ]
İsim:
license.txt
Boyut:
1.71 KB
Biçim:
Item-specific license agreed upon to submission
Açıklama:
İndir

Koleksiyon

2) Makale
WOS İndeksli Yayınlar Koleksiyonu