Improved catalytic activity of aspergillus oryzae β-galactosidase by covalent immobilization on eupergit cm

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dc.contributor.authorAslan, Yakup
dc.contributor.authorTaher, Alan Yaseen
dc.contributor.authorCavidoğlu, İsa
dc.date.accessioned2019-11-28T23:26:10Z
dc.date.available2019-11-28T23:26:10Z
dc.date.issuedDescember, 2018en_US
dc.departmentMühendislik Fakültesien_US
dc.description.abstractIn this study, Aspergillus oryzae β-Galactosidase (AOG) was immobilized onto Eupergit CM. By optimizing the immobilization conditions such as pH and molarity of immobilization buffer, enzyme/support ratio and duration of immobilization, 100.00% immobilization yield and 129.82% activity yield was achieved. The optimum temperature (55 °C) of free enzyme was not changed while optimum pH of free enzyme was shifted from 4.5 to 5.5 after immobilization. Kinetic constants for free and immobilized enzyme were also determined by using the Lineweaver-Burk plot. The Km values of the free and immobilized enzymes were determined to be 307.7 and 234.2 g / L respectively, while the Vmax values were determined to be 0.366 g D-Glucose / L.min and 0.415 g D-Glucose / L.min respectively. The operational and storage stabilities of immobilized enzyme were also studied. The activity of immobilized enzyme decreased to 99.3% after repeated twenty usage while decreased to 98.3% after fifteen days of storage. Further, the immobilized enzyme was used for the hydrolyzing the cow's milk lactose. By using the immobilized enzyme, the milk lactose was completely hydrolyzed in four hours. Consequently, immobilized AOG can be used in the industrial production of lactose-free cow's milk.en_US
dc.description.sponsorshipSiirt Üniversitesi BAP Koordinatörlüğü Proje No: 2017-SİÜFEB-010en_US
dc.identifier.endpage1655en_US
dc.identifier.issue6en_US
dc.identifier.scopus2-s2.0-85057212441
dc.identifier.scopusqualityQ3
dc.identifier.startpage1648en_US
dc.identifier.urihttp://www.thejaps.org.pk/docs/v-28-06/15.pdf
dc.identifier.urihttps://hdl.handle.net/20.500.12604/2066
dc.identifier.volume28en_US
dc.indekslendigikaynakScopus
dc.language.isoenen_US
dc.relation.ispartofThe Journal of Animal & Plant Sciences
dc.relation.publicationcategoryMakale - Uluslararası - Editör Denetimli Dergien_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.rightsAttribution-NonCommercial-NoDerivs 3.0 United States*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/3.0/us/*
dc.snmzKG_20241224
dc.subjectAspergillus oryzae; β-Galactosidase; covalent immobilization; Eupergit CM; lactose-free cow's milk; lactose intoleranceen_US
dc.titleImproved catalytic activity of aspergillus oryzae β-galactosidase by covalent immobilization on eupergit cmen_US
dc.typeArticleen_US

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