Purification and characterization of thermostable ?-amylase from thermophilic Anoxybacillus flavithermus

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dc.authoridMatpan Bekler, Fatma/0000-0001-8253-9568
dc.contributor.authorFincan, S. Aguloglu
dc.contributor.authorEnez, B.
dc.contributor.authorOzdemir, S.
dc.contributor.authorBekler, F. Matpan
dc.date.accessioned2024-12-24T19:25:23Z
dc.date.available2024-12-24T19:25:23Z
dc.date.issued2014
dc.departmentSiirt Üniversitesi
dc.description.abstractThis study reports on the purification and characterization of thermostable alpha-amylase (alpha-1-4 D-glucan glucanohydrolase EC 3.2.1.1) from a newly isolated Anoxybacillus flavithennus. A. flavithermus was used, which was isolated from hot water springs of Omer, Afyonkarahisar, Turkey. The gram-positive, spore-forming, motile, moderately thermophilic bacteria were found to be a strain of A. flavithermus analysed by 16S rRNA comparison. The optimal conditions for bacterial growth were determined to be at 20th h, 55 degrees C and pH 6.0. Maximum alpha-amylase activity was obtained at 55 degrees C at pH 7.0 after 24h of incubation. Thermostable alpha-amylase from A. flavithermus was purified by 70% (NH4)(2)SO4 and ion-exchange chromatography (5.2-fold; 65.8% yield). The molecular weight of alpha-amylase was 60 kDa, as estimated by sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE). The alpha-amylase hydrolyzed soluble starch at 55 degrees C with K-m: 0.005 mM and V-max: 3.5 mu mol min(-1). (C) 2013 Elsevier Ltd. All rights reserved.
dc.identifier.doi10.1016/j.carbpol.2013.10.048
dc.identifier.endpage150
dc.identifier.issn0144-8617
dc.identifier.issn1879-1344
dc.identifier.scopus2-s2.0-84890152872
dc.identifier.scopusqualityQ1
dc.identifier.startpage144
dc.identifier.urihttps://doi.org/10.1016/j.carbpol.2013.10.048
dc.identifier.urihttps://hdl.handle.net/20.500.12604/6394
dc.identifier.volume102
dc.identifier.wosWOS:000331779600019
dc.identifier.wosqualityQ1
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.language.isoen
dc.publisherElsevier Sci Ltd
dc.relation.ispartofCarbohydrate Polymers
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.snmzKA_20241222
dc.subjectAnoxybacillus flavithermus
dc.subjectalpha-Amylase
dc.subjectThermostable
dc.subjectPurification
dc.titlePurification and characterization of thermostable ?-amylase from thermophilic Anoxybacillus flavithermus
dc.typeArticle

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