Comparison of newly developed hydroxyl-functionalized monodisperse HILIC columns new HILIC column

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dc.contributor.authorDinc, Saliha
dc.contributor.authorOlmez, Saba Samatya
dc.contributor.authorTuncel, Ali
dc.date.accessioned2024-12-24T19:28:15Z
dc.date.available2024-12-24T19:28:15Z
dc.date.issued2017
dc.departmentSiirt Üniversitesi
dc.description.abstractIn this study, new hydroxyl-functionalized monodisperse polymeric hydrophilic interaction chromatography (HILIC) columns were developed using different derivatization agents. In addition, the influences of derivatization temperature of the best agent and polymer composition on the separation were investigated under HILIC conditions. Monodisperse-porous hydrophilic particles were synthesized by the seeded polymerization method using 3-chloro-2-hydroxypropile methacrylate (HPMA-CL) and ethylene glycol dimethacrylate (EGDMA) monomers. The chloropropyl terminal ends of the poly(HPMA-Cl-co-EGDMA) particles were derivatized with amine group of ethanolamine (EA), diethanolamine, and triethanolamine (TEA) at 80 degrees C through nucleophilic reaction. The performance of synthesized particles was evaluated with the amount of ligand on the particle surfaces, column backpressure, and separation power under HILIC condition. TEA was found to be the best derivatization agent for the separation of toluene, acrylamide, thymine, adenine, and cytosine in respect to resolution factors (>1.5 for all analytes) and theoretical plate numbers (64.562N/m for acylamide). Upon determination of the best ligand, then the effect of different derivatization temperatures and polymer composition on TEA performance was investigated. Of all the tested polymer compositions, the chromatographic performance of TEA-M-80 (the derivatization of TEA at 80 degrees C together with M polymer composition) was found to be the best.
dc.identifier.doi10.1080/10826076.2017.1343731
dc.identifier.endpage655
dc.identifier.issn1082-6076
dc.identifier.issn1520-572X
dc.identifier.issue12
dc.identifier.scopus2-s2.0-85029505504
dc.identifier.scopusqualityQ3
dc.identifier.startpage649
dc.identifier.urihttps://doi.org/10.1080/10826076.2017.1343731
dc.identifier.urihttps://hdl.handle.net/20.500.12604/6991
dc.identifier.volume40
dc.identifier.wosWOS:000414217300005
dc.identifier.wosqualityQ4
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.language.isoen
dc.publisherTaylor & Francis Inc
dc.relation.ispartofJournal of Liquid Chromatography & Related Technologies
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.snmzKA_20241222
dc.subjectDerivatization conditions
dc.subjectdifferent ligands
dc.subjecthydrophilic interaction chromatography
dc.subjecthydroxyl functionalized
dc.subjecttriethanolamine
dc.titleComparison of newly developed hydroxyl-functionalized monodisperse HILIC columns new HILIC column
dc.typeArticle

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