Investigation of the Effectiveness of Chromogenic Media in the Isolation of Pasteurella multocida and Mannheimia haemolytica from Calf Nasal Swab Samples

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dc.contributor.authorTel, Osman Yaşar
dc.contributor.authorÖtkün, Songül
dc.contributor.authorYücetepe, Ayfer Güllü
dc.contributor.authorKeskin, Oktay
dc.date.accessioned2024-12-24T19:18:17Z
dc.date.available2024-12-24T19:18:17Z
dc.date.issued2022
dc.departmentSiirt Üniversitesi
dc.description.abstractAccurate and rapid diagnosis of disease agents is the most important step in terms of control practices. Chromogenic media are culture media that allow the formation of colonies in colors specific to target microorganisms. Because they are target-specific, they do not require valida- tion of results but provide ease of use and time savings. In this study, it was aimed to evaluate the effectiveness of a chromogenic medium (Pasteurella BDR kit) in the detection of Pasteurella multocida and Mannheimia haemolytica agents in the Pasteurelleceae family, which cause respi- ratory disease in cattle. In this study, nasal swab samples taken from calves showing symptoms of respiratory disease were cultured in chromogenic and standard media. Suspicious growing colonies were confirmed by polymerase chain reaction for P. multocida and M. haemolytica. While 31 (36.9%) samples formed colonies with the chromogenic medium in the color specific to the target bacteria, 28 (33.3%) samples were positively determined for the target bacteria using the standard cultural method. The results of 26 samples were positive by both cultural diagnosis methods. When the results were compared with the traditional cultural diagnosis, agreement was found to be 92.86%. All colored colonies grown on the chromogenic medium were also tested by polymerase chain reaction (PCR). It was determined that the chromogenic medium detected P. multocida at a rate of 92.86% (n = 26) and M. haemolytica at a rate of 100% (n = 2) by forming colo- nies with a family-specific color. As a result, it was concluded that the use of chromogenic media is beneficial in the practical, rapid, and high-accuracy diagnosis of target agents.
dc.identifier.doi10.5152/VetSciPract.2022.221830
dc.identifier.endpage86
dc.identifier.issn2822-3608
dc.identifier.issue3
dc.identifier.startpage81
dc.identifier.trdizinid1175919
dc.identifier.urihttps://doi.org/10.5152/VetSciPract.2022.221830
dc.identifier.urihttps://search.trdizin.gov.tr/tr/yayin/detay/1175919
dc.identifier.urihttps://hdl.handle.net/20.500.12604/5047
dc.identifier.volume17
dc.indekslendigikaynakTR-Dizin
dc.language.isoen
dc.relation.ispartofVeterinary sciences and practices (Online)
dc.relation.publicationcategoryMakale - Ulusal Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.snmzKA_20241222
dc.subjectMikrobiyoloji
dc.titleInvestigation of the Effectiveness of Chromogenic Media in the Isolation of Pasteurella multocida and Mannheimia haemolytica from Calf Nasal Swab Samples
dc.typeArticle

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