IDENTIFICATION OF Prunus persica ROOTSTOCKS TO RESISTANT TO ROOT-KNOT NEMATODES USING MARKER ASSISTED SELECTION

Basit Öğe Kaydı
dc.contributor.authorBasbuga, Selcuk
dc.contributor.authorBasbuga, Sevil
dc.contributor.authorCan, Canan
dc.contributor.authorAtli, Halit Seyfettin
dc.date.accessioned2024-12-24T19:33:17Z
dc.date.available2024-12-24T19:33:17Z
dc.date.issued2022
dc.departmentSiirt Üniversitesi
dc.description.abstractPrunus is an important genus in the Prunoideae subfamily of the Rosaceae family. Some species such as peach (Prunus persica), almond (Prunus dulcis), plum (Prunus cerasifera, Prunus salicina, Prunus domestica) are used as rootstocks in stone fruit production because they can adapt to harsh climate and environmental conditions. It is aimed to produce rootstocks resistant to pathogens with classical and genetic breeding studies. Root knot nematodes slow down the active growth of Primus fruit trees such as peach and almond, reduce the resistance and cause yield loss. Recently, the use of nematicides in controling root knot nematodes has been restricted and that the damage has reached greater dimensions. For this reason, it has gained importance to use root-knot nematode-resistant rootstocks against nematodes in Prunus species. DNA marker systems that show tight linkage to genes (RMia, Ma and RMja genes) that provide resistance to root knot nematodes in Prunus species have been developed and it has been reported that these systems can be used with marker assisted selection (MAS) studies. In this study, resistance against root knot nematodes of F-1 hybrid individuals obtained from Ferragnes x GN22, Nemaguard x Ferragnes, GN22 x AB3, Nemaguard x AB3, Ferragnes x Nemaguard, Nemaguard x GN22 were determined. Three markers have been identified in the parent individuals [Nemaguard, GN22, Ferragnes, AB3 (Amygdalus orientalis Mill.) and GF67] that provide polymorphic discrimination, thus allowing selection of Ft individuals. As a result of PCR analyzes performed with these markers; 82 hybrid individuals were tested with the AMPP117 primer, and it was determined that 64 individuals were resistant and 18 individuals exhibited a sensitive profile. Additionaly, 77 hybrid individuals were screened with the LRR65 primer where 29 individuals were resistant and 48 individuals exhibited a sensitive profile. Totally, 86 hybrid individuals were tested with the SSRO5I12 primer, and that 34 individuals were resistant and 52 individuals exhibited a sensitive profile. As a result, the selection of peach hybrids resistant to root knot nematodes was achieved by using the markers whose usability was reported in marker assisted selection.
dc.description.sponsorshipScientific and Technological Research Council of Turkey (TUBITAK) [217O024]
dc.description.sponsorshipThe authors thank to the Scientific and Technological Research Council of Turkey (TUBITAK) for supporting this study with project number 217O024, and the Directorate of the Pistachio Research Station (Gaziantep, Turkey) for providing plant materials.
dc.identifier.endpage10747
dc.identifier.issn1018-4619
dc.identifier.issn1610-2304
dc.identifier.issue11
dc.identifier.startpage10738
dc.identifier.urihttps://hdl.handle.net/20.500.12604/8049
dc.identifier.volume31
dc.identifier.wosWOS:000892066900021
dc.identifier.wosqualityN/A
dc.indekslendigikaynakWeb of Science
dc.language.isoen
dc.publisherParlar Scientific Publications (P S P)
dc.relation.ispartofFresenius Environmental Bulletin
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.snmzKA_20241222
dc.subjectRoot-knot nematodes
dc.subjectResistance peach rootstock
dc.subjectSSR markers
dc.subjectMarker assisted selection
dc.titleIDENTIFICATION OF Prunus persica ROOTSTOCKS TO RESISTANT TO ROOT-KNOT NEMATODES USING MARKER ASSISTED SELECTION
dc.typeArticle

Dosyalar

Koleksiyon

WOS İndeksli Yayınlar Koleksiyonu