Analysis of mirna in plants by next generation sequencing technology

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dc.contributor.authorEren, Abdil Hakan
dc.contributor.authorİlhan, Emre
dc.contributor.authorİnal, Behçet
dc.date.accessioned2024-12-24T19:09:47Z
dc.date.available2024-12-24T19:09:47Z
dc.date.issued2016
dc.departmentSiirt Üniversitesi
dc.description.abstractmiRNAs (microRNAs) find in the intron sites of the genes. miRNAs are produced from their gene region and non-coding RNAs. In plants, the production of miRNAs that consist of 21–24 nucleotides in length starts in nucleus and continues coordinately in cytoplasm. miRNAs help to protect the integrity of genome duo to define expression levels of genes related to development and stress in plant. In this way, miRNAs are considered to defend 30% of eukaryotic genomes. There are a few types of small RNAs such as miRNAs, siRNAs, phasiRNAs and NAT – siRNAs in plants. Various methods such as Northern Blotting, Flow cytometer, cloning, qRT – PCR, Microarray, sequencing and RNA-seq (transcriptome profiling) were developed to detect and discover functions of these small RNAs. Species and tissue specific miRNAs can be easily identified with this method called as miRNA-seq, one of the Next Generation Sequencing methods. In miRNA studies, the algorithms such as miRDeep-Seq, miRanalyzer, miRCat, miRExpress, miRTRAP can be used. This review summarizes about up to date applications of next generation seqeuncing technologies in miRNA-seq studies. © 2016, Centenary University. All rights reserved.
dc.identifier.endpage454
dc.identifier.issn1308-7576
dc.identifier.issue3
dc.identifier.scopus2-s2.0-85011850165
dc.identifier.scopusqualityQ3
dc.identifier.startpage448
dc.identifier.urihttps://hdl.handle.net/20.500.12604/3774
dc.identifier.volume26
dc.indekslendigikaynakScopus
dc.language.isotr
dc.publisherCentenary University
dc.relation.ispartofYuzuncu Yil University Journal of Agricultural Sciences
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.snmzKA_20241222
dc.subjectMiRNA biogenesis
dc.subjectNext Generation Sequencing
dc.subjectQRT-PCR
dc.subjectRNA-Seq
dc.titleAnalysis of mirna in plants by next generation sequencing technology
dc.title.alternativeBitkilerde yeni nesil dizileme teknolojisi ile miRNA analizi
dc.typeArticle

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