Effects of Thymoquinone On Erythrocyte Aggregation Kinetics: An In Vitro Study

dc.contributor.authorÜstünova, Savaş
dc.contributor.authorMeral, İsmail
dc.contributor.authorÜyüklü, Mehmet
dc.date.accessioned2024-12-24T19:18:26Z
dc.date.available2024-12-24T19:18:26Z
dc.date.issued2022
dc.departmentSiirt Üniversitesi
dc.description.abstractErythrocyte aggregation is associated with a series of physio-pathological processes in body and according to this, current study was designed to examine how the thymoquinone (TQ) and aggregation kinetics relation changes when the aggregation properties of erythrocyte suspensions are intervened using different approaches, such as changing the suspension environment or erythrocyte surface properties. Blood samples from 40 male Wistar albino rats (three months old weighing 250-300 g) were randomly divided into four groups, as 1) whole blood (control), 2) 1/2 dilution (plasma was diluted with PBS), 3) dextran 500 (1% dextran 500 was dissolved in plasma) and 4) glutaraldehyde. Blood samples were taken from abdominal aorta under anesthesia. The erythrocyte suspensions were incubated at 37°C for one hour in the absence or presence of TQ, with a final concentration of 1 mg/ml. Then, the electrical properties of erythrocyte suspensions were recorded, and the electrical capacitance was monitored using an inductance, capacitance, and resistance meter (LCR meter) at 100 kHz. It was found that TQ incubation decreased the aggregation index in control and dextran groups while increased it in 1/2 dilution and GA groups. It was also found that TQ incubation increased the time constant in whole blood and dextran while decreased it in 1/2 dilution and GA. The TQ can normalize the altered erythrocyte aggregation responses in both ways that is, it decreases the increased aggregation or increases the decreased aggregation. Therefore, it may have very critical role in rouleaux formation-induced circulatory disorders.
dc.identifier.doi10.5505/ejm.2022.65037
dc.identifier.endpage256
dc.identifier.issn1301-0883
dc.identifier.issn1339-3886
dc.identifier.issue2
dc.identifier.startpage250
dc.identifier.trdizinid1124292
dc.identifier.urihttps://doi.org/10.5505/ejm.2022.65037
dc.identifier.urihttps://search.trdizin.gov.tr/tr/yayin/detay/1124292
dc.identifier.urihttps://hdl.handle.net/20.500.12604/5118
dc.identifier.volume27
dc.indekslendigikaynakTR-Dizin
dc.language.isoen
dc.relation.ispartofEastern Journal of Medicine
dc.relation.publicationcategoryMakale - Ulusal Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.snmzKA_20241222
dc.subjectBiyoloji
dc.titleEffects of Thymoquinone On Erythrocyte Aggregation Kinetics: An In Vitro Study
dc.typeArticle

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