PAEP gene restriction fragment length polymorphism and its effects on milk composition in cross-bred Hamdani sheep

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dc.contributor.authorTurgut, Ali Osman
dc.contributor.authorGülendağ, Erman
dc.contributor.authorKoca, Davut
dc.contributor.authorÜner, Sefa
dc.date.accessioned2024-12-24T19:17:11Z
dc.date.available2024-12-24T19:17:11Z
dc.date.issued2024
dc.departmentSiirt Üniversitesi
dc.description.abstract?-lactoglobulin (?LG) stands as the primary whey protein in ruminant milk, synthesized by mammary gland cells during lactation and encoded by the ?LG gene. This study aimed to assess the impact of ?LG gene exon II polymorphism on milk composition traits in crossbreed Hamdani sheep. Sheep were examined for clinical diseases and mastitis. Milk and blood samples were only collected from healthy ewes. The composition and physical properties of milk were analyzed using milk autoanalyzer. The ?LG gene exon II region's 452 bp PCR products were subjected to RFLP analysis using the RsaI restriction enzyme. Two genotypes, AA and AB, were identified for the ?LG gene exon II region, with A and B allele frequencies of 0.7 and 0.3, respectively. Statistical analysis, conducted with Minitab® (Version: 19.2020.2.0), revealed that the AA genotype is associated with a higher milk fat percentage p<0.05). However, no significant genotype effect was observed for other milk composition traits in cross-bred Hamdani sheep. These results suggest that PAEP genotypes could serve as valuable indicators for enhancing milk composition in cross-bred Hamdani sheep through breeding programs.
dc.identifier.doi10.31797/vetbio.1402523
dc.identifier.endpage41
dc.identifier.issn2548-1150
dc.identifier.issue1
dc.identifier.startpage35
dc.identifier.trdizinid1233436
dc.identifier.urihttps://doi.org/10.31797/vetbio.1402523
dc.identifier.urihttps://search.trdizin.gov.tr/tr/yayin/detay/1233436
dc.identifier.urihttps://hdl.handle.net/20.500.12604/4831
dc.identifier.volume9
dc.indekslendigikaynakTR-Dizin
dc.language.isoen
dc.relation.ispartofJournal of advances in vetbio science and techniques
dc.relation.publicationcategoryMakale - Ulusal Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.snmzKA_20241222
dc.subjectPCR-RFLP
dc.subjectmilk fat
dc.subjectBeta-lactoglobulin
dc.subjectcross-bred Hamdani sheep
dc.subjectPAEP
dc.titlePAEP gene restriction fragment length polymorphism and its effects on milk composition in cross-bred Hamdani sheep
dc.typeArticle

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