The Isolation and Examination of the Host Specificity of Local Bacteriophages for Use Against Brucella abortus

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dc.contributor.authorÖtkün, Songül
dc.contributor.authorErdenlig, Sevil
dc.contributor.authorSaytekin, Ahmet Murat
dc.date.accessioned2024-12-24T19:17:07Z
dc.date.available2024-12-24T19:17:07Z
dc.date.issued2023
dc.departmentSiirt Üniversitesi
dc.description.abstractBacteriophages are viruses that infect bacteria. Although their effects on bacteria have been known for many years, the discovery of antibiotics has limited the widespread use of bacteriophages against bacterial infections. However, it is now essential to reconsider using phage therapies due to antimicrobial-resistant bacteria and uncontrolled bacterial zoonotic diseases becoming a global problem. This study aimed to isolate lytic phages against B. abortus, the most common agent that causes bovine brucellosis, which has become a worldwide animal and public health problem. This performed isolation is from cattle farms known to be previously or currently infected, and the study also evaluates the lytic effects of these phages on B. abortus, B. melitensis, B. suis biotypes, B. ovis and B. neotomae and B. abortus field isolates in our culture collection. In this process, seven local brucella-specific phages were identified by evaluating 112 samples via general phage isolation and purification. The lyticity of the isolated bacteriophages were analyzed with international reference: B. abortus (bv 1, 2, 3, 4, 5, 6, 9), B. melitensis (bv 1, 2, 3), B. suis (bv 1, 2, 3, 4, 5) biovars, B. ovis, B. neotomae and B. abortus field strains (n:20). It was found that 85% of B. abortus strains produced a lysis pattern like Tbilisi ? through local phages. In terms of the lysis results, three different B. abortus specific phages were isolated (98 ?, 104 ?, and (P35, P70, P94/1, P94/2, P94/3) phages). It is thought that the applying cocktails prepared from these phages to fight against brucellosis will significantly contribute to controlling the disease. Since 15% of the field isolates were found to be rough strains, it is recommended that R/C ? are included in the prepared cocktails.
dc.identifier.doi10.31196/huvfd.1293366
dc.identifier.endpage85
dc.identifier.issn2146-717X
dc.identifier.issn2146-7188
dc.identifier.issue1
dc.identifier.startpage80
dc.identifier.trdizinid1183104
dc.identifier.urihttps://doi.org/10.31196/huvfd.1293366
dc.identifier.urihttps://search.trdizin.gov.tr/tr/yayin/detay/1183104
dc.identifier.urihttps://hdl.handle.net/20.500.12604/4778
dc.identifier.volume12
dc.indekslendigikaynakTR-Dizin
dc.language.isoen
dc.relation.ispartofHarran Üniversitesi Veteriner Fakültesi Dergisi
dc.relation.publicationcategoryMakale - Ulusal Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.snmzKA_20241222
dc.subjectBrucellosis
dc.subjectBrucella abortus
dc.subjectBacteriophage
dc.subjectPhage therapy
dc.titleThe Isolation and Examination of the Host Specificity of Local Bacteriophages for Use Against Brucella abortus
dc.typeArticle

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