Inhibition Effects of Some Sulfonamides on Carbonic Anhydrase from European seabass (Dicentrachus labrax)

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dc.contributor.authorErdem, Deryanur
dc.contributor.authorYerlikaya, Emrah
dc.contributor.authorCeyhun, Saltuk Bugrahan
dc.contributor.authorDemirdag, Ramazan
dc.contributor.authorSenturk, Murat
dc.contributor.authorErdogan, Orhan
dc.contributor.authorKufrevioglu, Omer Irfan
dc.date.accessioned2019-11-18T12:10:16Z
dc.date.available2019-11-18T12:10:16Z
dc.date.issued2011
dc.departmentBelirleneceken_US
dc.description.abstractAlpha-carbonic anhydrase (EC: 4.2.1.1; CA) was purified from European seabass gill and liver. The purification procedure was composed of preparation of homogenate (or hemolysate) and affinity chromatography on Sepharose 4B-tyrosine-sulfanilamide. Some sulfonamides exhibited in vitro inhibitory effects on the enzyme activity. Ki constants and IC50 values for these drugs were determined by Lineweaver-Burk graphs and plotting activity % vs. [I], respectively. IC50 values of sulfanilamide, mafenide, acetazolamide, 2-amino-1,3,5-tiyadiazol-5 sulfonamide were 980 ??M, 142 ??M, 20 ??M and 34 ??M for gill carbonic anhydrase (GCA), 126 ??M, 23 ??M, 14 ??M and 2.58 ??M for liver carbonic anhydrase (LCA), respectively. Some sulfonamides exhibited much stronger inhibitory activity against GCA and LCA at low micromolar concentrations with the Ki values ranging from 0.21 to 76.0 ??M as compared with other CAs.en_US
dc.identifier.urihttps://hdl.handle.net/20.500.12604/1624
dc.language.isoenen_US
dc.relation.publicationcategoryUluslararası Hakemli Dergi Makalesien_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.snmz#KayıtKontrol#
dc.subjectCarbonic anhydrase (CA), European seabass, liver carbonic anhydrase (LCA), Gill carbonic anhydrase (GCA), inhibition, sulfonamide.en_US
dc.titleInhibition Effects of Some Sulfonamides on Carbonic Anhydrase from European seabass (Dicentrachus labrax)en_US
dc.typeArticleen_US

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