Mardin ili Artuklu ve Kızıltepe ilçelerinde yetiştirilen yerel nar genotiplerinin organik asit, fenolik bileşenler ve şeker içeriklerinin belirlenmesi
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Date
2022
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Journal ISSN
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Publisher
Siirt Üniversitesi
Access Rights
info:eu-repo/semantics/openAccess
Abstract
Bu çalışmada; Mardin ili Artuklu ve Kızıltepe ilçelerinde yetiştirilen 18 nar genotipinin şeker, organik asit ve fenolik bileşen içeriklerinin kromogrofik yöntemlerden HPLC yöntemi ile tespit edilmesi amaçlanmıştır. Araştırmada yer alan genotiplerin meyve suyu örneklerinde HPLC-RID cihazında yapılan şeker analizleri, HPLC-DAD cihazında yapılan organik asit analizleri ve HPLC cihazında yapılan fenolik bileşen analizleri sonucunda elde edilen bulgular istatiksel olarak analiz edilmiştir. Ortalamalar arasındaki farklılıkların önem seviyeleri (p<0.05) Duncan çoklu karşılaştırma testine göre yapılmıştır. Buna göre; nar genotiplerinin glikoz içeriği miktarının 96.84-60.33 g/L, fruktoz içeriği miktarının 86.22-60.53 g/L, sakkaroz içeriği miktarının 397.87-233.37 g/L ve ksiloz içeriği miktarının 169.96-114.14 g/L arasında değiştiği tespit edilmiştir. Genotiplerin, tartarik asit miktarının 11.09-0.30 g/L ve pürivik asit miktarının 1.65-0.53 g/L arasında değiştiği tespit edilmiştir. Genotiplerin fenolik bileşen içeriklerinden askorbik asit 7.78-3.64 mg/g, hidroksibenzoik asit 1.37–0.13 mg/g, gentisik asit 0.12-0.02 mg/g, ferrulik asit 9.76- 2.98 mg/g, o-kumarin 0.09-0.002 mg/g, neohesperidin 0.23-0.01 mg/g, kumarin 0.03-0.003 mg/g, resveratrol 0.14-0.033 mg/g, kuersetin 0.09-0.005 mg/g ve gallik asit miktarlarının 17.16-6.77 mg/g arasında değiştiği belirlenmiştir.
In this study; It was aimed to determine the sugar, organic acid and phenolic component contents of 18 pomegranate genotypes grown in Artuklu and Kızıltepe districts of Mardin province by HPLC method, one of the chromographic methods. In the fruit juice samples of the genotypes included in the study, the findings obtained as a result of sugar analyzes made in HPLC-RID device, organic acid analyzes made in HPLC-DAD device and phenolic component analyzes made in HPLC device were analyzed statistically. Significance levels of the differences between the means (p<0.05) were made according to Duncan multiple comparison test. According to this; It was determined that the glucose content of pomegranate genotypes varied between 96.84-60.33 g/L, fructose content 86.22-60.53 g/L, sucrose content between 397.87-233.37 g/L and xylose content between 169.96-114.14 g/L. It was determined that the genotypes ranged between 11.09-0.30 g/L of tartaric acid and 1.65-0.53 g/L of pyruvic acid. Among the phenolic component contents of the genotypes, ascorbic acid 7.78-3.64 mg/g, hydroxybenzoic acid 1.37-0.13 mg/g, gentisic acid 0.12-0.02 mg/g, ferrulic acid 9.76-2.98 mg/g, o-coumarin 0.09-0.002 mg/g, It was determined that the amounts of neohesperidin 0.23-0.01 mg/g, coumarin 0.03-0.003 mg/g, resveratrol 0.14-0.033 mg/g, quercetin 0.09-0.005 mg/g and gallic acid varied between 17.16-6.77 mg/g.
In this study; It was aimed to determine the sugar, organic acid and phenolic component contents of 18 pomegranate genotypes grown in Artuklu and Kızıltepe districts of Mardin province by HPLC method, one of the chromographic methods. In the fruit juice samples of the genotypes included in the study, the findings obtained as a result of sugar analyzes made in HPLC-RID device, organic acid analyzes made in HPLC-DAD device and phenolic component analyzes made in HPLC device were analyzed statistically. Significance levels of the differences between the means (p<0.05) were made according to Duncan multiple comparison test. According to this; It was determined that the glucose content of pomegranate genotypes varied between 96.84-60.33 g/L, fructose content 86.22-60.53 g/L, sucrose content between 397.87-233.37 g/L and xylose content between 169.96-114.14 g/L. It was determined that the genotypes ranged between 11.09-0.30 g/L of tartaric acid and 1.65-0.53 g/L of pyruvic acid. Among the phenolic component contents of the genotypes, ascorbic acid 7.78-3.64 mg/g, hydroxybenzoic acid 1.37-0.13 mg/g, gentisic acid 0.12-0.02 mg/g, ferrulic acid 9.76-2.98 mg/g, o-coumarin 0.09-0.002 mg/g, It was determined that the amounts of neohesperidin 0.23-0.01 mg/g, coumarin 0.03-0.003 mg/g, resveratrol 0.14-0.033 mg/g, quercetin 0.09-0.005 mg/g and gallic acid varied between 17.16-6.77 mg/g.
Description
Fen Bilimleri Enstitüsü, Bahçe Bitkileri Ana Bilim Dalı
Keywords
Ziraat, Agriculture
