Characterization of a Thermally Stable ?-galactosidase Produced by Thermophilic Anoxybacillus sp. AH1

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dc.contributor.authorAcer, Ömer Acer
dc.contributor.authorBekler, Fatma Matpan
dc.date.accessioned2024-12-24T19:18:10Z
dc.date.available2024-12-24T19:18:10Z
dc.date.issued2021
dc.departmentSiirt Üniversitesi
dc.description.abstractThermostable ?-galactosidases from thermophilic bacteria have attracted increasing interest to have various advantages in industrial and biotechnological applications. In this study, a highly thermally stable ?-galactosidase produced by Anoxybacillus sp. AH1was purified and characterized. The highest enzyme production was achieved after the bacterium was incubated for 24 hours. The enzyme was purified by precipitation with ammonium sulphate dialysis, gel filtration chromatography using Sephadex G-75. After the purification steps, ?-galactosidase was found to be purified 10.2-fold and a yield of 13.9%. The molecular mass of the galactosidase was estimated to be 75 kDa by SDS-PAGE. The purified enzyme was highly stable and retained at 71% of the original activity at 60 °C and 53% at 70 oC within 120 minutes. The Km and Vmax values of purified ?-galactosidase were calculated as 1.249 mM and 0.5 ?mol minutes-1, respectively. Ca2+, Zn2+, and Mg2+ significantly activated ?-galactosidase activity, whereas enzyme activity was inhibited significantly by Cu+2 as well as by the metal ion chelators1,10-phenanthroline (phen) and ethylenediaminetetraacetic acid (EDTA). The Purified ?-galactosidase activity was increased by PMSF (phenylmethylsulfonyl fluoride), PCMB (p-chloromercuribenzoic acid), DTT (dithiothreitol), and ?-ME (?-mercaptoethanol) at 2 mM, but inhibited completely by NEM (N-ethylmaleimide) at 1 mM.
dc.identifier.doi10.46810/tdfd.835632
dc.identifier.endpage136
dc.identifier.issn2149-6366
dc.identifier.issue1
dc.identifier.startpage130
dc.identifier.trdizinid437206
dc.identifier.urihttps://doi.org/10.46810/tdfd.835632
dc.identifier.urihttps://search.trdizin.gov.tr/tr/yayin/detay/437206
dc.identifier.urihttps://hdl.handle.net/20.500.12604/4990
dc.identifier.volume10
dc.indekslendigikaynakTR-Dizin
dc.language.isoen
dc.relation.ispartofTürk Doğa ve Fen Dergisi
dc.relation.publicationcategoryMakale - Ulusal Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.snmzKA_20241222
dc.subjectAnoxybacillus sp. AH1
dc.titleCharacterization of a Thermally Stable ?-galactosidase Produced by Thermophilic Anoxybacillus sp. AH1
dc.typeArticle

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