Purification of carbonic anhydrase-II from sheep liver and inhibitory effects of some heavy metals on enzyme activity

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dc.contributor.authorDemirdag, Ramazan
dc.contributor.authorYerlikaya, Emrah
dc.contributor.authorKufrevioglu, Omer Irfan
dc.date.accessioned2019-11-18T10:47:23Z
dc.date.available2019-11-18T10:47:23Z
dc.date.issued2012
dc.departmentBelirleneceken_US
dc.description.abstractIn this study; sheep carbonic anhydrase-II (SCA-II) (E.C: 4.2.1.1) was purified from sheep liver and in vitro effects of heavy metals on the enzyme was examined. SCA-II isozyme was purified with about 203 purification fold, a specific activity of 2320 EU/mg-protein and a yield of 72 by using Sepharose-4B-L tyrosine-sulfanilamide affinity gel chromatography. Purity of the SCA-II enzyme was verified by SDS-PAGE technique and subunit molecular mass of the enzyme was found as 29 kDa. In addition to this, inhibitory effects of some metal ions on the enzyme were examined. In this study, sheep liver tissue was chosen; because the liver is an organ in which metal wastes of air, water and food are collected and it is easy to obtain the liver tissue. Because of the very important duties of CA enzyme on living beings, the effect of metals on the CA enzyme was investigated.en_US
dc.identifier.urihttps://hdl.handle.net/20.500.12604/1618
dc.language.isoenen_US
dc.relation.publicationcategoryUluslararası Hakemli Dergi Makalesien_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.snmz#KayıtKontrol#
dc.subjectSCA-II, purification, metal, inhibitionen_US
dc.titlePurification of carbonic anhydrase-II from sheep liver and inhibitory effects of some heavy metals on enzyme activityen_US
dc.typeArticleen_US

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