Covalent immobilization of Aspergillus oryzae ?-galactosidase and Bacillus licheniformis protease with Amino-Multi Walled Carbon Nanotubes

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dc.contributor.authorTaher, Alan Yaseen
dc.contributor.authorAlizadeh, Mohammad
dc.contributor.authorAslan, Yakup
dc.date.accessioned2024-12-24T19:10:09Z
dc.date.available2024-12-24T19:10:09Z
dc.date.issued2024
dc.departmentSiirt Üniversitesi
dc.description.abstractThis study was carried out with the aim of covalent immobilization of Aspergillus oryzae beta-galactosidase and Bacillus licheniformis protease on multi-walled amino-carbon nanotubes. In this method, fractional 2k design was used to study the effect of seven continuous factors (activation pH, glutaraldehyde molarity, activation time, buffer solution pH, buffer solution molarity, MWCNT-NH3-glutaraldehyde amount and stabilization time) on the stabilization efficiency and enzyme activity. . Design-expert software was used to analyze data and draw graphs. The results showed that the aforementioned factors predict the level of enzyme activity of Bacillus licheniformis protease and Aspergillus oryzae beta-galactosidase with correlation coefficients of 0.80 and 0.92 at the rate of 77 and 88%, respectively. Also, the correlation coefficient of the covalent fixation efficiency model of Aspergillus oryzae beta-galactosidase and Bacillus licheniformis protease on multi-walled carbon nanotubes was 0.89 and 0.82, respectively, and the studied factors were able to determine the covalent fixation beta efficiency, respectively. Aspergillus oryzae galactosidase and Bacillus licheniformis protease on multi-walled amino-carbon nanotubes predict 83 and 77%, respectively. © 2024 Tarbiat Modares University. All rights reserved.
dc.identifier.doi10.22034/FSCT.20.145.208
dc.identifier.endpage224
dc.identifier.issn2008-8787
dc.identifier.issue145
dc.identifier.scopus2-s2.0-85184893002
dc.identifier.scopusqualityQ4
dc.identifier.startpage208
dc.identifier.urihttps://doi.org10.22034/FSCT.20.145.208
dc.identifier.urihttps://hdl.handle.net/20.500.12604/3961
dc.identifier.volume20
dc.indekslendigikaynakScopus
dc.language.isoen
dc.publisherTarbiat Modares University
dc.relation.ispartofJournal of Food Science and Technology (Iran)
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.snmzKA_20241222
dc.subjectAlkaline protease
dc.subjectcarbon nanotubes
dc.subjectcovalent immobilization
dc.subject?-galactosidase
dc.titleCovalent immobilization of Aspergillus oryzae ?-galactosidase and Bacillus licheniformis protease with Amino-Multi Walled Carbon Nanotubes
dc.typeArticle

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