The Effects of Grape Seed Extract on The Some Enzymes and Metabolites in Diabetic Rats
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Tarih
2018
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
In this study, the effects of Grape Seed Extract (GSE) on glucose, enzymatic activities of elastase and collagenase, and certain biochemical parameters, were investigated. 32 female Wistar albino rats were used in the process. The subjects were randomly divided into 4 groups (control, diabetic (DM), GSE, DM+GSE). A single dose of saline was injected intraperitoneally to the rats in the control group and a single dose of streptozotocin (STZ, 45 mg/kg) was administered intraperitoneally to the rats in the diabetic group. In the GSE group, GSE (0.6 ml/rat) was administered daily via intragastric tube for 20 days, and DM+GSE group received a single dose of STZ intraperitoneally along with 0.6 ml GSE/rat. After 72 hours, blood samples were taken from all the rats. Rats with a fasting blood glucose level of 250 mg/dL and more were considered to be diabetic. At the end of the experiment, blood samples were taken again and were sent for elastase, collagenase, glucose, urea, total protein, cholesterol, and AOPP analysis. Glucose levels in diabetic rats were 671 mg/dL, compared to the glucose level of 335 mg/dL in the DM+GSE group. Total protein levels decreased in the DM+GSE group; the levels of glucose, urea, cholesterol and ascorbic acid also demonstrated a significant difference (p<0.001). No significant change was detected in the AOPP levels. The activities of collagenase (p<0.01) and elastase (p<0.05) in the DM group had moderate increases with respect to the control. It can, therefore, be surmised that the GSE reduces glucose levels, inhibits production of urea, regulates renal function, prevents possible nephropathy, overcomes albuminuria and alleviates protein loss, increases vitamin C levels and minimally affects collagenase and elastase activities.
Açıklama
Anahtar Kelimeler
Tıbbi Araştırmalar Deneysel,Endokrinoloji ve Metabolizma,Veterinerlik
Kaynak
Van Veterinary Journal
WoS Q Değeri
Scopus Q Değeri
Cilt
29
Sayı
3
