Determination of antimicrobial susceptibility and virulence-related genes of Trueperella pyogenes strains isolated from various clinical specimens in animals

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dc.contributor.authorGulaydin, O.
dc.contributor.authorKayikci, C.
dc.contributor.authorGulaydin, A.
dc.date.accessioned2024-12-24T19:30:47Z
dc.date.available2024-12-24T19:30:47Z
dc.date.issued2024
dc.departmentSiirt Üniversitesi
dc.description.abstractIn this study, a total of 32 Trueperella pyogenes strains isolated from different disease specimens in cattle, sheep and goats were examined. Antimicrobial susceptibility of the isolates to 10 antimicrobials were determined using the E -test method and MIC values of the antimicrobials were investigated. The genes that play a role in the antimicrobial resistance or virulence of T. pyogenes were determined by PCR using gene specific primers. In the study, all the isolates were susceptible to penicillin and cephalosporin. The highest resistance rate in the isolates was determined against streptomycin (56.25%) and tetracycline (53.12%) and MIC 90 values for these antimicrobials were found to be >256 mu g/ml and 12 mu g/ml, respectively. The ermX gene was found to be positive in 8 (80%) of 10 isolates that were resistant to macrolide group antimicrobials. Among 20 aminoglycoside resistant isolates, aadA1 , aadA9 , strA-strB , and aac(6')-aph(2'') genes were determined in 5 (25%), 14 (70%), 7 (35%) and 1 (5%) of the isolates, respectively. When the presence of virulence -related genes in the isolates was examined, nanP (93.75%), fimA (93.75%) and plo (90.62%) genes were detected in the majority of the isolates. While the cbpA gene was negative in all isolates, the fimG gene was found in a limited number of the isolates (15.62%). It was concluded that streptomycin and tetracycline resistance should be considered in T. pyogenes isolates. Also, nanP , fimA and plo genes may have an important role in the pathogenesis of the infections.
dc.description.sponsorshipResearch Fund of Siirt University [2021-SIUVET-031]
dc.description.sponsorshipAcknowledgements This study was supported by the Research Fund of Siirt University with the project number 2021-SIUVET-031. A summary of this study was presented as a poster at the 15th Veterinary Micro- biology Congress.
dc.identifier.doi10.24425/pjvs.2024.149349
dc.identifier.endpage202
dc.identifier.issn1505-1773
dc.identifier.issn2300-2557
dc.identifier.issue2
dc.identifier.startpage193
dc.identifier.urihttps://doi.org/10.24425/pjvs.2024.149349
dc.identifier.urihttps://hdl.handle.net/20.500.12604/7679
dc.identifier.volume27
dc.identifier.wosWOS:001254281800004
dc.identifier.wosqualityN/A
dc.indekslendigikaynakWeb of Science
dc.language.isoen
dc.publisherPolska Akad Nauk, Polish Acad Sciences, Univ Warmia & Mazury Olsztyn
dc.relation.ispartofPolish Journal of Veterinary Sciences
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.snmzKA_20241222
dc.subjectantimicrobial susceptibility Trueperella pyogenes
dc.subjectvirulence -related genes
dc.titleDetermination of antimicrobial susceptibility and virulence-related genes of Trueperella pyogenes strains isolated from various clinical specimens in animals
dc.typeArticle

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