Molecular Investigation and Phylogenetic Analysis of Ehrlichia canis in Dogs in Siirt, Türkiye

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dc.contributor.authorÇelik, Burçak Aslan
dc.contributor.authorYılmaz, Ali Bilgin
dc.contributor.authorÇelik, Özgür Yaşar
dc.contributor.authorAyan, Adnan
dc.contributor.authorKılınç, Özlem Orunç
dc.contributor.authorÖzdemir, Ramazan
dc.contributor.authorAyan, Özge Oktay
dc.date.accessioned2024-12-24T19:16:45Z
dc.date.available2024-12-24T19:16:45Z
dc.date.issued2022
dc.departmentSiirt Üniversitesi
dc.description.abstractEhrlichia canis is the primary etiologic agent of canine monocytic ehrlichiosis, a tick-transmitted disease of dogs. The aim of this study is to molecularly investigate the presence of E. canis and to reveal its prevalence in dogs in Siirt province. The animal material of the study is consisted of a total of 82 dogs. A region of the 16S ribosomal RNA gene of E. canis was targeted for PCR amplification. As a result of the conducted Nested-PCR, positivity was detected at the rate of 10.53% (4/38) in male dogs and 13.64% (6/44) in females, and Ehrlichia canis specific bands of size 389 bp were obtained in 10 (12.20%) dogs in total. The phylogenetic tree was constructed with the Maximum Likelihood (MCL) method, The nucleotide sequence was registered in the NCBI GenBank database with access numbers OK331365.1-OK331366. Early detection of the disease by means of hematological, serological, or molecular tests is very important in terms of prognosis. More studies should be performed to determine vector-disease relationships in this region about ticks that vector the disease.
dc.identifier.doi10.24925/turjaf.v10i10.1832-1837.5338
dc.identifier.endpage1837
dc.identifier.issn2148-127X
dc.identifier.issue10
dc.identifier.startpage1832
dc.identifier.trdizinid1175878
dc.identifier.urihttps://doi.org/10.24925/turjaf.v10i10.1832-1837.5338
dc.identifier.urihttps://search.trdizin.gov.tr/tr/yayin/detay/1175878
dc.identifier.urihttps://hdl.handle.net/20.500.12604/4552
dc.identifier.volume10
dc.indekslendigikaynakTR-Dizin
dc.language.isoen
dc.relation.ispartofTürk Tarım - Gıda Bilim ve Teknoloji dergisi
dc.relation.publicationcategoryMakale - Ulusal Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.snmzKA_20241222
dc.subjectTıbbi Araştırmalar Deneysel,Patoloji,Mikrobiyoloji,Jeokimya ve Jeofizik,Parazitoloji,Viroloji,Jeoloji,Tıbbi Laboratuar Teknolojisi
dc.titleMolecular Investigation and Phylogenetic Analysis of Ehrlichia canis in Dogs in Siirt, Türkiye
dc.typeArticle

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