IMPROVED CATALYTIC ACTIVITY OF ASPERGILLUS ORYZAE ?-GALACTOSIDASE BY COVALENT IMMOBILIZATION ON EUPERGIT CM

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dc.authoridaslan, yakup/0000-0001-9668-9559
dc.contributor.authorAslan, Y.
dc.contributor.authorTaher, A. Y.
dc.contributor.authorCavidoglu, I
dc.date.accessioned2024-12-24T19:33:12Z
dc.date.available2024-12-24T19:33:12Z
dc.date.issued2018
dc.departmentSiirt Üniversitesi
dc.description.abstractIn this study, Aspergillus oryzae beta-Galactosidase (AOG) was immobilized onto Eupergit CM. By optimizing the immobilization conditions such as pH and molarity of immobilization buffer, enzyme/support ratio and duration of immobilization, 100.00% immobilization yield and 129.82% activity yield was achieved. The optimum temperature (55 degrees C) of free enzyme was not changed while optimum pH of free enzyme was shifted from 4.5 to 5.5 after immobilization. Kinetic constants for free and immobilized enzyme were also determined by using the Lineweaver-Burk plot. The K-m values of the free and immobilized enzymes were determined to be 307.7 and 234.2 g / L respectively, while the V-max values were determined to be 0.366 g D-Glucose / L.min and 0.415 g D-Glucose / L.min respectively. The operational and storage stabilities of immobilized enzyme were also studied. The activity of immobilized enzyme decreased to 99.3% after repeated twenty usage while decreased to 98.3% after fifteen days of storage. Further, the immobilized enzyme was used for the hydrolyzing the cow's milk lactose. By using the immobilized enzyme, the milk lactose was completely hydrolyzed in four hours. Consequently, immobilized AOG can be used in the industrial production of lactose-free cow's milk.
dc.description.sponsorshipSiirt University Scientific Research Projects Coordinatorship
dc.description.sponsorshipThe authors acknowledge Rohm and Haas and Bio-Cat Companies for Eupergit CM and Fungal lactase, respectively. The authors also acknowledge Siirt University Scientific Research Projects Coordinatorship for their financial support.
dc.identifier.endpage1655
dc.identifier.issn1018-7081
dc.identifier.issn2309-8694
dc.identifier.issue6
dc.identifier.startpage1648
dc.identifier.urihttps://hdl.handle.net/20.500.12604/8021
dc.identifier.volume28
dc.identifier.wosWOS:000450417000015
dc.identifier.wosqualityN/A
dc.indekslendigikaynakWeb of Science
dc.language.isoen
dc.publisherPakistan Agricultural Scientists Forum
dc.relation.ispartofJournal of Animal and Plant Sciences-Japs
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.snmzKA_20241222
dc.subjectAspergillus oryzae
dc.subjectbeta-Galactosidase
dc.subjectcovalent immobilization
dc.subjectEupergit CM
dc.subjectlactose-free cow's milk
dc.subjectlactose intolerance
dc.titleIMPROVED CATALYTIC ACTIVITY OF ASPERGILLUS ORYZAE ?-GALACTOSIDASE BY COVALENT IMMOBILIZATION ON EUPERGIT CM
dc.typeArticle

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