Comparison of newly developed hydroxyl- functionalized monodisperse HILIC columns new HILIC column

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dc.contributor.authorDinç, Saliha
dc.contributor.authorSamatya Ölmez, Saba
dc.contributor.authorTuncel, Ali
dc.date.accessioned2019-11-08T07:37:02Z
dc.date.available2019-11-08T07:37:02Z
dc.date.issued2017
dc.departmentBelirleneceken_US
dc.description.abstractIn this study, new hydroxyl-functionalized monodisperse polymeric hydrophilic interaction chromatography (HILIC) columns were developed using different derivatization agents. In addition, the influences of derivatization temperature of the best agent and polymer composition on the separation were investigated under HILIC conditions. Monodisperse–porous hydrophilic particles were synthesized by the seeded polymerization method using 3-chloro-2-hydroxypropile methacrylate (HPMA-CL) and ethylene glycol dimethacrylate (EGDMA) monomers. The chloropropyl terminal ends of the poly(HPMA-Cl-coEGDMA) particles were derivatized with amine group of ethanolamine (EA), diethanolamine, and triethanolamine (TEA) at 80°C through nucleophilic reaction. The performance of synthesized particles was evaluated with the amount of ligand on the particle surfaces, column backpressure, and separation power under HILIC condition. TEA was found to be the best derivatization agent for the separation of toluene, acrylamide, thymine, adenine, and cytosine in respect to resolution factors (>1.5 for all analytes) and theoretical plate numbers (64.562 N/m for acylamide). Upon determination of the best ligand, then the effect of different derivatization temperatures and polymer composition on TEA performance was investigated. Of all the tested polymer compositions, the chromatographic performance of TEA-M-80 (the derivatization of TEA at 80°C together with M polymer composition) was found to be the best.en_US
dc.identifier.issn1082-6076
dc.identifier.urihttps://hdl.handle.net/20.500.12604/1310
dc.language.isoenen_US
dc.publisherJournal of Liquid Chromatography & Related Technologiesen_US
dc.relation.publicationcategoryUluslararası Hakemli Dergi Makalesien_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.snmz#KayıtKontrol#
dc.titleComparison of newly developed hydroxyl- functionalized monodisperse HILIC columns new HILIC columnen_US
dc.typeArticleen_US

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