Comparison of newly developed hydroxyl- functionalized monodisperse HILIC columns new HILIC column
Yükleniyor...
Tarih
2017
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Journal of Liquid Chromatography & Related Technologies
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
In this study, new hydroxyl-functionalized monodisperse polymeric hydrophilic interaction chromatography (HILIC) columns were developed using different derivatization agents. In addition, the influences
of derivatization temperature of the best agent and polymer composition on the separation were
investigated under HILIC conditions. Monodisperse–porous hydrophilic particles were synthesized by the
seeded polymerization method using 3-chloro-2-hydroxypropile methacrylate (HPMA-CL) and ethylene
glycol dimethacrylate (EGDMA) monomers. The chloropropyl terminal ends of the poly(HPMA-Cl-coEGDMA) particles were derivatized with amine group of ethanolamine (EA), diethanolamine, and
triethanolamine (TEA) at 80°C through nucleophilic reaction. The performance of synthesized particles
was evaluated with the amount of ligand on the particle surfaces, column backpressure, and separation
power under HILIC condition. TEA was found to be the best derivatization agent for the separation of
toluene, acrylamide, thymine, adenine, and cytosine in respect to resolution factors (>1.5 for all analytes)
and theoretical plate numbers (64.562 N/m for acylamide). Upon determination of the best ligand, then
the effect of different derivatization temperatures and polymer composition on TEA performance was
investigated. Of all the tested polymer compositions, the chromatographic performance of TEA-M-80
(the derivatization of TEA at 80°C together with M polymer composition) was found to be the best.
