Carbonic Anyhdrase Enzyme From The Siirt Mohair Goat Liver : Purification, Characterization and Assessment of Enzyme Kinetics Against Metal Toxicity

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dc.contributor.authorYerlikaya, Emrah
dc.contributor.authorKarageçili, Hasan
dc.contributor.authorDemirdağ, Ramazan
dc.contributor.authorKaya, Mustafa Oğuzhan
dc.date.accessioned2024-12-24T19:16:05Z
dc.date.available2024-12-24T19:16:05Z
dc.date.issued2017
dc.departmentSiirt Üniversitesi
dc.description.abstractBecause of their physiological and clinical roles, carbonic anhydrases (CAs) are the most studied enzymes. In earlier studies; CA enzymes have been purified and characterized from the tissues and erythrocytes of many organisms such as; dog, swine, sheep, chicken, bee, fish, bovine, bacteria and human. In this study, the CA enzyme has purified from Siirt Mohair Goat liver tissue with 1930.84 EU.mg-1 of specific activity, 57.28% of purification yield and 80.55 of purification folds. The purity of the purified enzyme has confirmed by SDS-PAGE. As the characterization of CA enzyme’s in Siirt Mohair Goat liver has been done; the optimum ionic strength: 25 mM, the optimum pH: 8.0, the optimum temperature: 40ºC and the stable pH: 7.0 has been determined. Inhibitory effects of some metal ions have been examined on the purified CA enzyme. IC50 values of inhibiting metal ions were found as 2.24, 2.76, 2.36, 3.20, 2.55, 2.25, 3.28, 2.13, 3.10, 1.75, 2.16 and 3.50 mM for Al3+, Ni2+, Cd2+, Cu2+, Pb2+, Ba2+, Zn2+, B3+, Fe3+, Se2+, Ag+ and Co2+ respectively. As a result, CA enzyme was first purified from the Siirt Mohair Goat liver and the characteristics of the enzyme were investigated in this study.
dc.identifier.doi10.15671/HJBC.2018.206
dc.identifier.endpage634
dc.identifier.issn1303-5002
dc.identifier.issn2687-475X
dc.identifier.issue4
dc.identifier.startpage629
dc.identifier.trdizinid286734
dc.identifier.urihttps://doi.org/10.15671/HJBC.2018.206
dc.identifier.urihttps://search.trdizin.gov.tr/tr/yayin/detay/286734
dc.identifier.urihttps://hdl.handle.net/20.500.12604/4219
dc.identifier.volume45
dc.indekslendigikaynakTR-Dizin
dc.language.isoen
dc.relation.ispartofHacettepe Journal of Biology and Chemistry
dc.relation.publicationcategoryMakale - Ulusal Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.snmzKA_20241222
dc.subjectGenetik ve Kalıtım,Biyokimya ve Moleküler Biyoloji
dc.titleCarbonic Anyhdrase Enzyme From The Siirt Mohair Goat Liver : Purification, Characterization and Assessment of Enzyme Kinetics Against Metal Toxicity
dc.typeArticle

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