LHRH fusion protein immunization alters testicular development, ultrasonographic and histological appearance of ram testis

dc.contributor.authorÜlker, Hasan
dc.contributor.authorYılmaz, Ayhan
dc.contributor.authorKarakuş, Ferda
dc.contributor.authorYörük, Macit
dc.contributor.authorBudağ, Cemal
dc.contributor.authorde AVILA, David M
dc.contributor.authorReeves, Jerry J
dc.date.accessioned2019-11-10T10:49:05Z
dc.date.available2019-11-10T10:49:05Z
dc.date.issued2009
dc.departmentBelirleneceken_US
dc.description.abstractThis study was designed to evaluate the effectiveness of a luteinizing hormone-releasing hormone (LHRH) fusion protein immunization on reproductive traits in ram lambs including the changes in histology and ultrasonographic appearance of the testis. Thirty native ram lambs at 19 weeks of age were divided into control (C, n = 10), immunization (I, n = 10) and castration (E, n = 10) groups. Animals in immunization group were immunized against LHRH using ovalbumin-LHRH-7 (OL) protein generated by recombinant DNA technology as a primary and a booster injection at 19 and 23 weeks of age respectively. Animals were bled via jugular venepuncture at 2-week intervals to determine LHRH antibody and testosterone concentrations. Bi-weekly ultrasonographic examination of the testes was performed to determine the changes in ultrasonographic appearance as the age increased. Biopsied testicular tissues taken at 19, 29 and 41 weeks age were also evaluated. At 41 weeks of age, animals were slaughtered. Semen and epididymis were evaluated for the presence of sperm cells. Testicular development and sperm production were suppressed in the immunized animals. Seminiferous tubule diameters decreased, basal membrane of the tubule was thickened and hyalinized in immunized ram lambs. While testes of control animals gained their normal ultrasonographic appearance as the age increased, immunized animals had uniform hypoechogenic testicular structure as observed at 19 weeks of age until slaughter. Simultaneous histological and ultrasonographic evaluations indicated that the changes in testicular histology could partly be monitored via ultrasonographic imaging. Nevertheless, it is difficult to claim that ultrasonographic image reflects the exact changes in such instances. In conclusion, these results indicate that recombinant OL fusion protein is effective in immunocastration in ram lambs and has a potential to be used as an alternative to physical castration. Further research studies should be conducted to help assess reproductive status of testes from ultrasound images.en_US
dc.identifier.issn1439-0531
dc.identifier.urihttps://hdl.handle.net/20.500.12604/1325
dc.language.isoenen_US
dc.publisherReproduction in Domestic Animalsen_US
dc.relation.ispartofseries44: 593-599;
dc.relation.publicationcategoryUluslararası Hakemli Dergi Makalesien_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.snmz#KayıtKontrol#
dc.subjectLHRH, testisen_US
dc.titleLHRH fusion protein immunization alters testicular development, ultrasonographic and histological appearance of ram testisen_US
dc.typeArticleen_US

Dosyalar

Orijinal paket
Listeleniyor 1 - 1 / 1
Yükleniyor...
Küçük Resim
İsim:
LHRH fusion protein immunization alters testicular development, ultrasonographic and histological appearance of ram testis.pdf
Boyut:
388.34 KB
Biçim:
Adobe Portable Document Format
Lisans paketi
Listeleniyor 1 - 1 / 1
[ X ]
İsim:
license.txt
Boyut:
1.71 KB
Biçim:
Item-specific license agreed upon to submission
Açıklama:

Koleksiyon