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Öğe Examination of paraffin-embedded testes of domestic cats and dogs by light and scanning electron microscopy(Elsevier BV, 2025-06) Banu Kandil; Beste Demirci; Alev Gürol Bayraktaroğlu; Emre DemirciThis study was designed to determine the appropriate section thickness for SEM analysis of paraffin-embedded testes. This study used paraffin-embedded testes from 6 adult cats and 6 adult dogs. Tissue blocks were cut into 10, 20, and 40 µm thick sections and analyzed by SEM. In addition, seminiferous tubule and lumen diameter and germinative epithelium height were measured in 10 and 20 µm thick paraffin sections. Measurements from 10 and 20 µm thick paraffin sections showed that the height of the germinative epithelium and the diameter of the lumen and tubules were similar between cats and dogs (p > 0.05). In cats and dogs, tubule diameter and germinative epithelium height were higher in the 10 µm thick paraffin sections compared to the 20 µm thick paraffin sections. However, in both species, the lumen diameter was greater in the 20 µm thick paraffin sections than in the 10 µm thick paraffin sections (p < 0.05). Unlike 40 µm thick paraffin sections, 10 and 20 µm thick paraffin sections allowed the structural features of the testes to be examined. Additionally, 10 µm thick paraffin sections were ideal for histometric measurements, while 20 µm thick paraffin sections were suitable for detailed examination of late spermatids. The present study showed that testes may be examined in detail by SEM from 10 and 20 µm thick paraffin sections. In addition, this study may contribute to the design of new studies to reveal the missing three-dimensional structures of paraffin-embedded testicular tissues stored in tissue archives by analyzing them by SEM.Öğe Immunohistochemical Distribution of Heat Shock Proteins 27, 60, 70 and 90 in the Placenta of Goats in the Second and Third Trimester of Pregnancy(Wiley, 2025-02-27) Banu KandilDetermining the immunoexpression of HSPs in the placenta may contribute to the understanding of pregnancy physiology and immunotolerance mechanisms. This study aimed to determine the distribution of HSP27, HSP60, HSP70 and HSP90 in the placenta of goats. Tissue samples were taken from the placentomal and interplacentomal regions of the pregnant uterus of 12 goats, comprising 6 s and 6 third trimesters. Following routine histological procedures, immunohistochemical staining was performed on tissue sections. In the interplacentomal region, HSP27, HSP60, HSP70 and HSP90 in luminal and glandular epithelial, stromal and smooth muscle cells did not show differences between the second and third trimesters (p > 0.05). In the placentomal region, HSP27 and HSP60 in syncytial plaques and HSP27, HSP60, HSP70, and HSP90 in maternal stromal cells did not change as the pregnancy progressed (p > 0.05). There was no difference in HSP27, HSP60 and HSP90 in fetal stromal cells between the second and third trimesters (p > 0.05). HSP27 and HSP90 were positive in both trophoblast cells, HSP60 was positive in binucleate trophoblast cells, and HSP70 was positive in mononucleate trophoblast cells. In the third trimester compared with the second trimester, HSP27 was decreased (p < 0.05), while HSP90 in mononucleate and binucleate trophoblast cells did not show a difference (p > 0.05). HSP70 did not change in mononucleate trophoblast cells (p > 0.05), but HSP60 was increased in binucleate trophoblast cells (p < 0.05) as the pregnancy progressed. In conclusion, this study showed that HSPs had similar immunoexpression patterns in the interplacentomal region but different immunoexpression patterns in the placentomal region of the goat placentaÖğe Sexual Dimorphism of Foetal Sheep Skulls During the Second and Third Periods of Pregnancy.(2025-03) Güzel, Barış Can; Işbilir, FatmaThe skull is a very important structure, and it is the centre of many vital functions. There have been many studies on the skulls of mammals, but not many studies on the prenatal period. The aim of this study is to examine developmental sheep foetal skulls from the last two trimesters of pregnancy. A total of 40 sheep foetuses, 20 in the 2nd trimester (10 females and 10 males) and 20 in the 3rd trimester (10 females and 10 males), were examined. On the basis of CT scans of foetal skulls, morphometric measurements were performed by creating a three-dimensional (3D) model. Total skull length was statistically significant between males and females in the third trimester (p < 0.01). In the second trimester, the tooth length parameter was statistically significant between males and females (p < 0.01). In the second trimester, M3 was found to be statistically significant in the sheep foetus mandible (p < 0.01). It was determined that there was developmental sexual dimorphism between males and females.Öğe 3D Geometric Morphometric Analysis of Calcaneal Morphology in Domestic Caprinae: Sheep (Ovis aries) and Goat (Capra hircus)(MDPI AG, 2025-02-14) Barış Can Güzel; Tomasz Szara; Burak Ünal; Sokol Duro; Fatma İşbilir; Funda Yiğit; Mihaela-Claudia Spataru; Karolina Goździewska-Harłajczuk; Ozan GündemirThe calcaneus plays a critical role in balance, locomotion, and muscle attachment, making it a key structure for biomechanical adaptations. This study examined interspecies differences between sheep and goats, as well as intraspecies variations among different sheep breeds, in calcaneus morphology. A total of 128 right calcanei were analyzed using 3D geometric morphometric methods to allow detailed assessments of calcaneus shape and size. Among sheep, the Hamdani sheep displayed the largest calcaneus among the breeds, distinguishing them significantly from the others. In contrast, goats had a bilaterally narrower calcaneal body, while sheep featured a more pronounced distal portion that articulates with the talus and a deeper articular surface. Additionally, the sustentaculum tali were more developed in sheep. The bilaterally compressed calcaneal body in goats likely reflects their agility and ability to navigate steep, rocky terrains. In contrast, sheep's broader and more robust calcaneus may support their grazing lifestyle on flatter terrains, emphasizing their stability and weight-bearing capacity. These findings highlight the functional significance of calcaneal morphology in the locomotor strategies and physical capabilities of goats and sheep, providing valuable insights for comparative anatomy and veterinary science.Öğe Skull morphology in native and non-native cattle breeds in Türkiye.(2025-02-23) Gündemir, Ozan; Manuta, Nicoleta; Güzel, Barış Can; Bakıcı, Caner; Duro, Sokol; Ünal, Burak; Çakar, Buket; Szara, TomaszThis study aims to investigate morphological differences in the skulls of cattle breeds with different functional roles and geographical origins and to examine skull shape variations among breeds using geometric morphometrics methods. To this end, 95 skulls from Native (Eastern Anatolian Red and Southern Anatolian Red) and Non-native (Holstein and Simmental) breeds raised in Türkiye were analyzed. The results show a significant size difference between Native Breeds and Non-native Breeds, with the Non-native Breeds having larger skulls. The results also indicate considerable shape differences between Native and Non-native Breeds. No significant shape differences were observed between the two Native Breeds, which have shared the same geography for many years. Although no statistically substantial size difference was found between Simmental and Holstein, the nuchal region showed distinct shape differences. The nuchal part of the Simmental skull was more pronounced than other specimens. In native breeds, there was a more balanced proportion between the facial and neurocranial regions compared to the other two breeds. In Holstein skulls, the facial region was more pronounced in comparison to the neurocranial region, while in Simmental skulls, the neurocranial region was more prominent. This study provides valuable insights into the morphological characteristics of different cattle breeds, contributing to veterinary anatomy, biology, and paleontology.Öğe Shape and Size Variations in the Astragalus of Large and Small Bovids(MDPI AG, 2025-02-03) Burak Ünal; Barış Can Güzel; Buket Çakar; Yeşim Aslan Kanmaz; Funda Yiğit; Ozan Gündemir; Mihaela-Claudia SpataruThe astragalus acts as a crucial intermediary in the tarsal joint, facilitating the transfer of body weight to the lower extremities while also ensuring both stability and mobility through its articulation with adjacent bones, enabling efficient movement. This study investigates the morphological diversity of the astragalus in three modern cattle breeds, one goat breed, and three sheep breeds using three-dimensional geometric morphometric methods. A total of 225 specimens were analyzed in this study. Large bovids, such as the Holstein and Simmental cattle breeds, had a more developed lateral edge of the proximal trochlea and a more rounded astragalus. In small bovids, the Akkaraman and Morkaraman sheep breeds displayed a more prominent medial edge and a rounded astragalus form, while the Hair goat breed exhibited a rectangular structure with a well-developed lateral projection. The results of this study demonstrate that the astragalus serves as a reliable morphological marker for differentiating between large and small bovids, as well as between closely related taxa such as sheep and goats. These shape variations, particularly observed in the proximal trochlea, reflect functional adaptations to size, body mass, and locomotor demands, making the astragalus a critical element for taxonomic identification and biomechanical analysis.Öğe Distribution of Heat Shock Proteins 27, 60, 70, 90 in Testis and Epididymis of the Domestic Cats (Felis catus) and Dogs (Canis lupus familiaris).(Wiley, 2025-02) Banu Kandil; Alev Gürol BayraktarogluThis study aimed to examine the immunoexpression of HSP27, HSP60, HSP70, and HSP90 in the testis and epididymis of domestic cats (Felis catus) and dogs (Canis lupus familiaris). Testis and epididymis tissues from 6 adult cats and 6 adult dogs were used in this study. Immunohistochemical staining was done to determine the expression of HSPs. In cats and dogs, while HSP60 was detected only in Leydig cells, HSP90 was determined only in spermatogonia. HSP27 was observed only in smooth muscle cells of blood vessels. HSP70 was not detected in spermatocytes, spermatids, Leydig cells, or Sertoli cells, whereas HSP70 was determined in peritubular myoid cells. In addition, unlike cats, HSP70 was observed in spermatogonia of dog testes. HSP27 was determined in basal cells of the epididymal epithelium and smooth muscle cells of the ductal wall in all sections of the epididymis. However, no HSP60 was observed in the epididymis. While HSP70 was not detected in the epididymis of the cats, HSP70 was observed in basal cells of all sections of the epididymis of the dogs. While the epididymal epithelial cells showed HSP90 immunoreactivity in all parts of the epididymis, the smooth muscle cells of the ductal wall exhibited HSP90 immunoreactivity only in the cauda epididymidis. The findings of this study indicate that HSP27, HSP60, HSP70, and HSP90 exhibit different immunoexpression patterns in the testis and epididymis of cats and dogs and that these proteins play important roles in maintaining the reproductive functions of cats and dogs.Öğe Assessment of the effect of sodium tetraborate on oxidative stress, inflammation, and apoptosis in lead-induced nephrotoxicity(Taylor & Francis Ltd, 2024) Yaman, Turan; Akkoyun, H. Turan; Akkoyun, Mahire Bayramoglu; Karagozoglu, Fatma; Melek, Sule; Keles, Omer Faruk; Bengu, Aydin SukruExposure to Pb, a toxic heavy metal, is a risk factor for renal damage. Borax, an essential trace element in cellular metabolism, is a naturally occurring compound found in many foods. This study investigated the effects of sodium tetraborate (ST), a source of borax, on renal oxidative stress and inflammation in rats exposed to Pb. Wistar Albino rats (n = 24) were divided into four groups: Control (0.5 mL, i.p. isotonic), Pb (50 mg/kg/day/i.p.), ST (4.0 mg/kg/day/oral), and Pb + ST groups. At the end of the five-day experimental period, kidney tissue samples were obtained and analyzed. Histopathologically, the Pb-induced damage observed in the Pb group improved in the Pb + ST group. Immunohistochemically, Pb administration increased the expression of inducible nitric oxide synthase, cyclooxygenase-2, and caspase-3. When evaluated biochemically, Pb application inhibited catalase and glutathione peroxidase (GSH-Px) enzyme activities and activated superoxide dismutase enzyme activity. An increase in malondialdehyde levels was considered an indicator of damage. ST application increases glutathione peroxidase enzyme activity and decreased malondialdehyde levels. These results indicate that ST might play a protective role against Pb-induced renal damage via the upregulation of renal tissue antioxidants and cyclooxygenase-2, inducible nitric oxide synthase, and caspase-3 immunoexpression.Öğe Antioxidant properties of Ferulago angulata and its hepatoprotective effect against Nnitrosodimethylamine- induced oxidative stress in rats(2017)ABSTRACT Context: Ferulago angulata (Schlecht.) Boiss. (Apiaceae) (FASB) is used to treat liver diseases and has been used both as food and therapeutics by many cultures for thousands of years because of the natural antioxidant compounds. Objective: This study determines antioxidant properties of FASB flowers, the levels of minerals and vitamins, and also, evaluates the hepatoprotective effect of flowers against N-nitrosodimethylamine (NDMA) induced on liver tissue by assessing antioxidant enzymes and histopathological parameters in Wistar albino rats. Materials and methods: In the study, the rats were divided into six groups of ten. Control, untreated animals were given 0.9% NaCl. Rats were intraperitoneally given NDMA (10 mg/kg) for the first 7 days. FASB methanol extract (150 and 300 mg/kg) was administered orally for 21 days. Results: a-Tocopherol, retinol, ascorbic acid, total antioxidant activity, phenolic and flavonoid contents of FASB were 0.70 ± 0.13, 0.29 ± 0.03 lg/g, 139.32 ± 7.06 lg/100 g, 171.61 ± 6.05mM ascorbic acid/g, 90.47 ± 4.11mg GA/g and 37.39 ± 2.85mg QE/g. DPPH and hydroxyl radical scavenging activity was obtained IC50 67.34 ± 4.14 and 64.87 ± 4.68 lg/mL, respectively. Discussion and conclusion: The results of the study indicated that FASB flowers contain high levels of vitamins, minerals, total antioxidant activity, phenolics and flavonoids. Due to the positive effect on significant changes in antioxidant enzymes of liver tissue and histopathological examination, it is thought that the plant could be used as a hepatoprotective.Öğe Investigation of Some Biochemical Parameters in Sheep Naturally Infected with Cystic Echinococcosis(2019) İrak, Kıvanç; Çelik, Özgür Yaşar; Aslan Çelik, Burçak; Bolacalı, Memiş; Mert, Handan; Mert, NihatThis study was performed to reveal the changes in certain serum biochemical parameters in animals diagnosed with Cystic Echinococcosis. The material of the study consisted of 40 sheep aged 1-3, which were brought for slaughter from different animal farms of Siirt. Overall, 20 sheep which were determined to be healthy in physical examinations and had no pathological lesions in their internal organs were determined as the control group, while the remaining 20 sheep with cystic lesions in their lungs and livers which were protoscolex-positive formed the infected group. While no significant relevant difference between the K, Cl, Ca, Mg, GGT, ALT, ALB, TRIG and CHOL levels of health and infected animals (p>0.05), statistically meaningful changes in Na (p<0.01), TBIL (p<0.05), and TP and GLOB (p<0.001) levels and AST (p<0.001) activity were observed between the groups. As a result, it was determined that the changes in AST, total protein, globulin, and total bilirubin levels could be used as supportive laboratory analyzes in a determination of the prognosis of animals infected with Cystic Echinococcosus and of the physiopathologic alterations occurring in their livers.Öğe Effect of dietary yeast autolysate on performance, slaughter, and carcass characteristics, as well as blood parameters, in quail of both genders(2017) Bolacalı, Memiş; İrak, KıvançThis study was conducted ta determine the effects of dietary yeast autolysate (Saccharomyces cerevisiae) on performance, slaughter and carcass characteristics, as well as blood parameters, in Japanese quail of both genders. A total of 1000 (500 males and 500 females) one-day-old Japanese quail (Coturnix coturnix japonica) were randomly allocated ta one control group and four dietary groups (supplemented with 1, 2, 3, and 4% yeast autolysate) per gender, each containing 100 quail. Each dietary group was then divided into five replicate groups of 20 chicks. During the study (from 1 st ta 42nd day), quails fed dietary treatments supplemented with yeast autolysate had higher live bodyweight (LBW) and average daily live weight gain (ADG) than the control group, and the dietary supplementation of 2% yeast autolysate reduced feed intake (Fi) and feed conversion rate (FCR) far both genders. The highest carcass yield was observed in trial 1 % in male quail (P <0.01 ), and the control of female (P <0.05). The lowest abdominal fat percentage was observed in trial 1 % and 2% of male (P <0.05), and trials 2% and 3% in female quail (P <0.01). The highest breast percentage was observed in the trial 2% of female quail. Cholesterol was significantly lower in trial 2% of male (P <0.001) on day 42. Additionally, aspartate aminotransferase (AST) (P <0.001 ), alkaline phosphatase (ALP) (P <0.001) and albumin (ALB) (P <0.05) concentrations in male quail were statistically different among the groups on day 42. in general, good performance and reduced abdominal fat percentage and cholesterol level were observed in the group supplemented with 2% yeast autolysate. in this study, it was concluded that the addition of 2% yeast autolysate ta diet could be used as a performance enhancer far quail in the first 42 days of life.Öğe EFFECT OF CARBON TETRACHLORIDE (CCl4) AND ELLAGIC ACID ON RAT ERYTHROCYTE G6PD, 6PGD, GR, GST AND TrxR ENZYME ACTIVITIES(2019) AKKOYUN H.TURAN; Akkoyun MahireThe aim of this study was to investigate the effects of carbon tetrachloride and quercetin on selected trace element levels such as Cr (Chromium), Mn (Manganese), Fe (Iron), Cu (Copper) in rats. In the study, 28 male Wistar albino rats (200 +/- 300 g) were used. Rats were divided into 4 groups as Control, CCl4 (carbon tetrachloride), Qu (quercetin), Qu + CCl4 (quercetin + carbon tetrachloride) (n=7 in each group). When Cr (mu g/g) amount was examined an increase was observed in Qu administered group (p <0.01) compared to the control. It was increased in Qu (p <0,05) and CCl4 + Qu (p <0,001) treated groups compared to CCl4 group. Mn decreased in CCl4 and CCl4 + Qu groups compared to control. Fe (mu g/g) content was increased in CCl4, CCl4 + Qu and Qu (p<0.01) groups compared to Control. An increase in Qu treated group compared to CCl4 applied group (p<0.05) was observed. The CCl4 + Qu group decreased compared to the Qu group (p<0.01). Cu content of CCl4 and Qu groups showed an increase whereas CCl4 + Qu applied showed a decrease compared to control. As a result; It is thought that quercetin may have an effect on important trace element levels such as Cr (Chromium) Mn (Manganese), Fe (Iron), Cu (Copper) in the case of oxidative stress caused by carbon tetrachloride.Öğe SERUM BIOCHEMICAL AND HISTOPATHOLOGICAL EXAMINATIONS OF SOME TISSUES OF LAMBS WITH MUSCULAR DYSTROPHY IN VAN(2016) YILDIRIM, Serkan; İRAK, Kıvanç; MERT, Handan; DOĞAN, İnci; MERT, NihatWhite muscle disease (WMD), is an important disease also known as ‘muscular dystrophy’ in the lamb and calf. White muscle disease is the result of degeneration of skeletal and cardiac muscles in lambs. Lambs mostly affected with the congenital form either born death or die a few day after birth. The disease is a manifestation of lack of selenium, vitamin E or both. Van and surrounding villiages were visited and lambs with WMD examined. The lambs with 3-10 days of age were used as research materials. Necropsy and gross examinations was performed to all lambs. The blood samples were analyzed for Vitamin E amount, Creatine kinase (CK), Aspartate aminotransferase (AST) and Lactate dehydrogenase (LDH) activities. The level of Vitamin E was decreased, but the other parameters significantly increased. In heart, chest and gluteal muscle lesions in lambs were found. Tissue samples were histopathologically examined. Muscular dystrophic calcification in necrotic areas as well as hyaline degeneration and Zenker necrosis were determined. In the calcified region mononuclear cell infiltration mainly macrophages, were observed. In conclusion in lamb with white muscle disease; the activities of enzymes related to muscle health were raised drastically. In gluteal, chest and especially in heart muscle the hyalin and Zenker degeneration were noted.Öğe INVESTIGATION OF ANTIOXIDANT COMPOUNDS IN FLUOROTIC SHEEP(2016) DOĞAN, İnci; MERT, Handan; İRAK, Kıvanç; MERT, NihatAbstract Fluorosis, a condition which usually affects the formation of bone and teeth in human and animals, is an important health problem in Van and Agri provinces. This study was performed to determine the levels and the changes of antioxidant compounds in fluorotic sheep. 30 fluorotic sheep and 20 healthy Morkaraman sheep of 3-4 years old were used as living research materials. The diagnosis of fluorosis was confirmed by clinical examinations. The urine fluoride level was determined. Blood of all animals was taken from vena jugularis by appropriate techniques and analyzed for glutathione peroxidase (GPx), glutathione (GSH), superoxide dismutase (SOD), malondialdehyde (MDA), sialic acid (SA) and lipid-bound sialic acid (LSA). The levels of these parameters in healthy and fluorotic group were: 1028-416.8 mU /ml, 23.23-50.16 mg/ dl, 9.25- 7.88 mU/ ml, 1.62-0.56 nmol/ ml, 51.19-46.33 mg/ dl and 9.77- 12.16 mg/dl, respectively. Urine fluoride (F) levels were 1.65 ppm in healthy and 23.84 ppm in fluorotic sheep groups. Statistical differences was found between the average values of healthy and fluorotic groups as p? 0.0001 in GPx and GSH, p?0.001 in MDA, p?0.05 in SA, p?0.01 in LSA and p?0.001 in urine F levels. No statistical differences were found in SOD levels. (p?0.05) The results obtained in this study indicate that important changes were found in antioxidant systems of fluorotic sheeps.Öğe THE LEVELS OF NITRITE, NITRATE AND VITAMIN D3 IN HUMAN WITH GASTRIC CANCER(2016) İRAK, Kıvanç; MERT, N; MERT, Handan; COMBA, Bahat; DOĞAN, İnci; YÖRÜK, İbrahim HakkıStomach cancer is one of the most observed in Van and surroundings. Dietary source of Vitamin D are hepfull to survive from cancer with low mortality rates. There are so many reports on the association between nitrate intake from food and gastric cancer risk and also nitrate intake from water. The blood samples were taken patients with stomach cancer admitted to Department of Medical Oncology in Faculty of Medicine in Van. Ranging from 45-76 years old of age 32 male, 15 female patients with a total 47 were used in the research. Stomach adenocarcenoma were diagnosed from all patients with endoscopic biopsy. Then the chest X-Ray, abdominal computed tomography, biochemical and heamatological analysis were done and magnetic resonance were used when it is necessary for grading. The changes of blood parameters after 3-4 cycles of chemotherapy compared to the values at the diagnosis time were analyzed. The blood samples were taken after chemotherapy and analyzed for nitrate, nitrite spectrophotometrically and vitamin D by HPLC. Comparing with control group, the nitrite levels were increased in cancer patients before and after the chemotherapy. But the levels of nitrate were high before chemothrapy then decreased after chemotherapy. Vit D levels of two groups were low then the controls. These three compounds were significantly important for gastric cancer formation in this cases.Öğe CA 19-9, CA 125, CEA, GLUCOSE, INSULIN AND ACTH LEVELS IN PATIENTS WITH GASTRIC CANCER(2016) İRAK, Kıvanç; MERT, Handan; DOĞAN SÖĞÜTLÜ, İnci; MERT, NihatGastric tumor remains a major health hazard during the decades. Generally it is the third most common cases and has high mortality rates. In the presented study it was aimed to investigate the serum levels of CA 19-9, CA 125, CEA, insulin, glucose and ACTH in patients with gastric cancer. The blood samples of 22 male (45-74 ages old), 12 female (50-75 years old) and 15 healthy subjects were taken before the breakfast and sera were seperated and all analyzed were done immediately. The CA 19-9, CA 125, CEA levels were found in healthy and patients with gastric cancer as 2.09-17.32 ng/ml, 6.94-63.21 U/ml, 9.31-49.71 U/ml respectively. The differences between the groups of these three parameters were statistically significant (p?0.01). The fasting blood glucose and insulin amount were found in normal and cancer cases as, 83.14- 98.33mg/dl (p?0.05), 10.22-8.17 µU/ml (p?0.05) respectively. ACTH levels were slightly higher in cancer patients compared with controls 58.31- 44.28 pg/ml (p?0.05). Tumor markers were increased in all patients with gastric cancer. Some of the patients had diabetic pattern but blood glucose levels over 120 mg/dl were only documented in 8 cases. The results were evaluated clinic pathologically. As conclusion all analyzed parameters were changed in gastric cancer cases. Insulin and ACTH hormones can be properly assessed during the various stages of cancer. As far, we can still use CA 19-9, CA 125, CEA as a reliable tumor markers in gastric cancer.Öğe DETERMINATION OF THE LEVEL OF SOME VITAMIN IN WHEATGRASS AND GRASS(2016) İRAK, Kıvanç; MERT, Handan; YÖRÜK, İbrahim Hakkı; DOĞAN SÖĞÜTLÜ, İnci; MERT, Nihat…Öğe Bazı yem ve gübre sanayi kimyasallarının hyalüronidaz üzerine in vitro etkileri(2016) Kaya, Mustafa Oğuzhan; Yerlikaya, Emrah; Özyazıc, Mehmet Arif; İrak, KıvançABSTRACT This study investigates in vitro effects of some chemicals, used as a spreading factor on therapeutic and various medical fields and applied frequently in forage and fertilizer industry on bovine testicular hyaluronidase (BTH) which is substantially effective in glucotechnological applications and delivery of artificial insemination. BTH was purified through ammonium sulfate precipitation method and affinity gel (Sepharose-4B-L- tyrosine -m-anisidine). The inhibiton effects of Fe2SO4, C2H4N4, K2SiF6, CH2N2, CH4N2O and indole-butyric acid were determined on purified BTH. IC50 values of these chemicals were found as 7.097, 5.775, 6.854, 5.317, 4.617, 4.265 ×10-2 mM respectively and at the end of research, indole-butyric acid (IBA) was found as the strongest inhibitory effect compound by the value of IC50 with 4.265×10-2 mM.Öğe Deneysel Diyabet Oluşturulan Sıçanlarda Böbreklerin Histolojik Olarak Değerlendirilmesi(2015) Muzaffer Aydın Ketani, Berfin Kadiroğlu, Zelal KarakoçDiyabetes Mellitus (şeker hastalığı), karbonhidrat, yağ ve protein metabolizmalarında bozuklukla karakterize, yüksek kan glikoz seviyeleri ile seyreden ve vücutta pek çok sistemi etkileyerek yaşam boyu devam eden bir hastalıktır. Diyabetin komplikasyonlarına bağlı dokularda değişiklikler meydana gelmektedir. Histopatolojik düzeyde araştırma yapmak amacıyla sıçanlarda deneysel diyabet oluşturuldu. Denekler kontrol ve diyabet grubu olarak ikiye ayrıldı. Deneysel diyabet oluşturulurken sitrat tamponda çözdürülmüş streptozotosin 50 mg/kg intraperitoneal tek dozda enjekte edildi. Her gruptan yedi denek 7. 14. ve 21. günlerde böbrekleri çıkarıldıktan sonra sakrifiye edildi. Alınan örnekler mikroskopta incelenmek üzere tespit aşamalarından geçirilerek parafinde gömüldü. Mikroskop altında kontrol grubu böbrek dokusu normal bulundu. Diyabet grubunda ise kapillar bazal membranda kalınlaşma, Bowman kapsülünde daralma görüldü. Tübüllerde ise epitel dökülmesi, vakuolizasyon ve atrofi tespit edildi.Öğe Kör Fare (Spalax ehrenbergi, Nehring, 1898) Kolonunda MUC1, MUC2 ve MUC5AC’nin Dağılımı(2016) Muzaffer Aydın Ketani, Zelal Karakoç, Şennur KetaniBirçok türde gastrointestinal kanal boyunca salgılanan müsinlerin profillerinin birbirinden farklı olduğu gösterilmiştir. Sunulan çalışmada kör farelerin kolonunun epitel ve kadeh hücrelerinde MUC1, MUC2 ve MUC5AC proteinlerinin ekspresyonlarını immunohistokimyasal olarak ortaya koymayı amaçladık. Bu çalışmada ortalama ağırlıkları 200-220 gr. arasında değişen 2 adet dişi ve 2 adet erkek yetişkin kör fare kullanıldı. Kör fare kolonundan alınan doku örnekleri, formol-alkol solusyonunda 18 saat süre ile tespit yapıldı. Kör fare kolonunda luminal ve kript epitel hücrelerinden MUC1, MUC2 ve MUC5AC’nin ekspresse olduğu ve kadeh hücrelerinde MUC2’nin daha baskın olduğu tespit edildi. Sonuç olarak, kör fare kolonunda luminal ve kript epitel hücreleri ile kadeh hücrelerinden MUC1, MUC2 ve MUC5AC’nin lokalize olduğu bulundu.
