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Öğe A Significant Need in the Medical Field: A New Method for Putrescine, Spermine and Spermidine Using High-Performance Liquid Chromatography (HPLC)(MDPI AG, 2025-03-21) Naci Ömer AlayuntThe analysis of polyamines such as putrescine, spermine, and spermidine is very important for the diagnosis and treatment of various diseases, especially in the field of medicine and health. However, HPLC analysis methods are very limited in the literature. This study is very important in terms of validation parameters such as the linearity, reproducibility, and standard operating curves of polyamines such as serum putrescine, spermine and spermidine. Therefore, we developed a modified method for the analysis of serum putrescine, spermine, and spermidine. This study is superior to indirect measurement methods and is important as a gold standard. In this study, a reproducible method was developed according to the standard putrescine, spermine, and spermidine working curves and other validation criteria for HPLC. Polyamine biosynthesis is an ancient metabolic pathway found in all living organisms and the homeostasis of polyamines is essential for cell survival. This new HPLC method is a first in the field of medicine in that it provides fast, accurate, and reliable results in serum putrescine, spermine, and spermidine analysis and is used in routine analyses in the diagnosis of some specific diseases.Öğe Evaluation of miRNA Profile and Its Relationship with Metabolic Disorders in Obese and Pre-Obese Patients(MDPI AG, 2025-04-17) Kürşat Kargün; Erhan Aygen; Mehmet Fatih Ebiloğlu; Naci Ömer Alayunt; Lütfiye Kadıoğlu DalkılıçObesity is a growing global public health concern, with its prevalence rapidly increasing in Turkey, leading to severe consequences. Genetic factors, particularly mutations in structural genes and microRNAs (miRNAs) involved in gene expression regulation have been widely investigated in obesity research. This study aimed to explore the role of obesity-associated miRNAs and their potential interaction with vascular response alterations. A total of 60 obese and pre-obese patients and 26 age- and sex-matched healthy controls from the General Surgery Department of Fırat University Medicine School were included. The expression levels of 93 miRNAs were analyzed in 86 samples using the Fluidigm Biomark RT-PCR system, with 5S RNA as the housekeeping gene. Significant differences were observed in weight, BMI, cholesterol, triglyceride levels, and lymphocyte counts between the groups (p < 0.0001). Several miRNAs, including hsa-miR-148a-3p, hsa-miR-503-3p, hsa-miR-34a-5p, and hsa-miR-199a-3p, were significantly downregulated in obese patients. Additionally, gender-specific differences in miRNA expression were identified. These findings indicate that miRNAs play a crucial role in obesity pathophysiology and could serve as potential biomarkers and therapeutic targets for obesity treatment.Öğe Investigation of antibacterial, antifungal, and antioxidant activities in novel poly(DEAEMA-co-EMA)/polyindole composites(Wiley, 2025-03-29) Safiye Elif Korcan; Cengiz Soykan; Burak Tüfekçi; Naci Ömer Alayunt; Şah İsmail ÇiviIn the current study, in the first stage, amphiphilic poly(2-diethylamino ethylmethacrylate-co-ethyl methacrylate) [Poly(DEAEMA-co-EMA)] was synthesized from the 2-diethylamino ethylmethacrylate (DEAEMA) and the ethyl methacrylate (EMA) using the free radical polymerization method. In the second stage, five series of composites were prepared at different concentrations using the indole conductive monomer in the presence of iron(III) chloride (FeCl3) using the in situ oxidative polymerization technique in poly(DEAEMA-co-EMA) copolymer. Scanning electron microscope and Atomic force microscope images proved the homogeneous structure of the composites, and Fourier transform infrared spectroscopy analysis of the composite has shown that it has been produced successfully. The antimicrobial activity of the prepared poly(DEAEMA-co-EMA)/PIN composites was evaluated. Moreover, antioxidant studies of the composites were carried out. The results showed that the enhanced bioactivity of poly(DEAEMA-co-EMA)/PIN(90%) composite. In this study, all poly(DEAEMA-co-EMA)/PIN composites and polymers showed antibacterial activity against Streptococcus mutans. PCs had more antifungal activity against Aspergillus flavus than PIN, Poly(DEAEMA), and poly(DEAEMA-co-EMA). The antimicrobial activity of PCs was enhanced by increasing the permeability of the cell membrane with DEAEMA. It was determined that the antibacterial activity increased in direct proportion to the indole concentration. OSI values of PIN, Poly(DEAEMA), and poly(DEAEMA-co-EMA), and PCs ranged between 1.3513 and 0.1183. Highlights: Conductive Polymer Composites are a special commercial polymer composite class due to their biological properties. Discusses morphological properties of poly(DEAEMA-co-EMA)/PIN composites. Summarizes antimicrobial and antioxidant activities of poly(DEAEMA-co-EMA)/PIN composites. Demonstrate the high antimicrobial activity of Poly(DEAEMA-co-EMA)/PIN composites against S. mutans. Determine that Poly(DEAEMA-co-EMA)/PIN composites have antioxidant capacity.
