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Öğe Dose-Dependent Application of Silver Nanoparticles Modulates Growth, Physiochemicals, and Antioxidants in Chickpeas (Cicer arietinum) Exposed to Cadmium Stress(American Chemical Society (ACS), 2025-02-05) Abeer Elhakem; Jiahao Tian; Hilal Yilmaz; Wenjing Mao; Lisong Shao; Sipan Soysal; Mohammad Faizan; Jian Gao; Pravej AlamThe present study was intended to investigate the effects of silver nanoparticles (Ag NPs) on chickpea plants grown in cadmium (Cd)-contaminated soil. Chickpea seeds sown in earthen pots (filled with soil) were subjected to Cd stress (100 μM) in the form of CdCl2 (10 mL) 10 days after sowing (DAS). Exogenous applications with Ag NP concentrations 50, 100, and 200 μM were used to observe their effects on Cd-stressed plants. Growth, biochemical, and stress parameters were studied. Results showed that Ag NPs positively affected plant growth and ameliorated the toxic effects of Cd stress. Plant height, fresh weight, dry weight, total carotenoid content, rubisco activity, and net photosynthetic rate (PN) were significantly decreased by Cd stress but enhanced by 28, 29, 31, 30, 33, and 35%, respectively, by foliar application of Ag NPs. Similarly, Ag NPs increased the activity of superoxide dismutase (61%), catalase (58%), and peroxidase (68%) and reduced the malondialdehyde (28%) and hydrogen peroxide (23%) in chickpea plants. Protein content was also increased by the application of Ag NPs (16%). Furthermore, the addition of Ag NPs decreased the plant Cd content. According to the current study, adding Ag NPs to plants under Cd stress improved their growth and photosynthesis by reducing Cd absorption and improving plant stress tolerance. © 2025 The Authors. Published by American Chemical Society.Öğe Enhancing Wheat Productivity and Reducing Lead Uptake Through Biochar, Bentonite, and Rock Phosphate Integration(MDPI AG, 2025-04-14) Mohamed S. Elshikh; Mona S. Alwahibi; Zaffar Malik; Ahmad Ali; Hassan Mehmood; Hafiz Tanvir Ahmad; Sipan Soysal; P. V. Vara Prasad; Ivica Djalovic; Bogdan DugalicHeavy metal (HMs) toxicity has severely impacted wheat production and is considered an emerging threat to human health due to bioaccumulation. The application of organic and inorganic amendments has proven effective in mitigating HM’s phytotoxicity by limiting their mobility in soil and plants. A pot experiment was conducted to evaluate the efficiency of biochar (BC), bentonite (BN), and rock phosphate (RP), both individually and in combination, in alleviating lead (Pb) toxicity and enhancing wheat growth, and physiological attributes. The present investigation revealed that BC, BN, RP, and their combined mineral biochar amendments (MBAs) at 1.5% level significantly enhanced wheat growth along with reducing DTPA-extractable Pb in soil by 30.0–49.8% and Pb uptake in roots by 15.7–37.5% and in shoots by 34.5–48.5%. Antioxidant enzymatic activities were improved, and stress indicators were reduced in roots and shoots of wheat under Pb stress, including hydrogen peroxide (H2O2) by 50.7 and 81.0%, malondialdehyde (MDA) levels by 16.0 and 74.9%, and proline content by 34.5 and 64.0%, respectively. The effectiveness of the treatments is described in descending order viz. MBA-1 > MBA-3 > MBA-2 > BC > RP > BN under Pb stress. In conclusion, the integration of biochar, bentonite, and rock phosphate is a promising strategy for sustainable and cleaner cereal crop production under heavy metal stress conditions.Öğe Nano-Priming as Seed Priming Technology for Sustainable Agriculture(Apple Academic Press, 2025-02-21) Fatih Çiğ; Rojin Özek; Murat Erman; Sipan Soysal; Özge Uçar; Zeki Erden; Mustafa Ceritoğlu; Çağdaş Can Toprak; Sönmez Özbey; Muhammad A. Raza; Javeed A. Lone; Saifullah Abro; Muhammad Arshad; Mehmet EfeAgriculture is threatened by climate change and by the depletion of resources and biodiversity. Exploring new farming methods is needed to increase crop production and ensure food quality and safety in a sustainable way. Nanotechnology is an emerging trend that contributes to sustaining agricultural production. Seed nano-priming helps improve seed germination, seedling emergence, growth, and yield by resisting various plant stresses. Nano-priming is a more effective method than any other seed preparation method. Studies have shown several benefits of using seed nano-priming. By regulating biochemical pathways and the balance between reactive oxygen species and plant growth hormones, nano-priming helps increase resistance against stresses and diseases with the limited use of pesticides and fertilizers.Moreover, nano-priming prevents the continued damage caused by conventional agriculture, resulting in an environmentally safer system for farmers and consumers. The present review provides an overview of developments in the field, showing the challenges and possibilities of using nanotechnology in seed nano-priming to contribute to sustainable farming practices.Öğe Wheat genotypes respond differently under polyethylene glycol (PEG) induced drought stress during germination and early seedling growth stages(Pakistan Journal of Botany, 2024-08-01) Mst Masuma Akhter; M.A. Hasan; M.M. Bahadur; M.R. Islam; Muhammad Aamir Iqbal; Walid Soufan; Khandakar Aurib; Tanjila Akhter; Sipan Soysal; Ayman Elsabagh; Elsabagh Heniesh; Mohammad Sohidul IslamWater scarcity has emerged as one of a critical environmental constriction that negatively affect wheat growth, development and yield in Bangladesh. Identification of drought tolerant genotypes is crucial at drought sensitive initial stages of plant growth especially germination and seedling growth stages. To cater this issue, 30 wheat genotypes were subjected to polyehelene glycol (PEG 6000) induced drought stress levels of 0,-2.0 and-4.0 Mpa (osmotic stresses) and their response was tested at the germination and seedling growth stages. The experimental set up was Completely Ramdomized Design (CRD) with five replications. The results revealed that the rate of germination (RG) among the genotypes varied significantly with the different water potential levels, and the RG decreased with the advancement of stress. The stress tolerance index (STI) values based on RG were > 0.900 in wheat cultivars of Shatabdi, BARI Gom 25, BAW 1118, BAW 1151, BAW 1161, E 2, E 18, E 30, E 34 and E 38under severe water deficit stress, while the values were < 0.800 in wheat genotypes BARI Gom 26, BARI Gom 27, BARI Gom 28, BAW 1130, BAW 1140, BAW 1143, BAW 1168, E 28 and E 42. The co-efficient of germination (COG) was significantly decreased with increasing water potential stresses (-2 &-4 bars), and the genotypes Sourav, BARI Gom 25, BAW 1118, BAW 1135, BAW 1151, BAW 1157, BAW 1161, BAW 1163, BAW 1170, BAW 1171, E 2, E 18, E 23, E 29, E 34 and E 38showed > 0.980 STI values whereas, BARI Gom 26, BARI Gom 27, E 24, E 28, E 42, BAW 1130, BAW 1140 and BAW 1168 produced < 0.970 STI values under higher water potential. Higher STI values (> 0.920) regarding the germination vigour index (GVI) under higher water stress were observed in BARI Gom 25, E 18, E 23, E 34, E 38, BAW 1118, BAW 1161 and BAW 1170, and very lower STI values (< 0.800) were obtained in the Shatabdi, BARI Gom 26, BARI Gom 27, BAW 1130, BAW 1140, BAW 1168, E 3, E 24, E 28 and E 42 genotypes. The genotypes Shatabdi, BARI Gom 25, E 18, E 38, BAW 1118, BARI Gom 27, E 24, E 34, BAW 1143 and BAW 1170 showed higher STI values (> 0.550) indicating tolerant genotypes and the genotypes Sourav, BARI Gom 26, BARI Gom 28, BAW 1130, BAW 1151, BAW 1168, E 2, E 3, E 28 and E 29demonstrated lower STI values (< 0.450) designating susceptible genotypes. As water deficit stress increased, the shoot weight (g) of wheat genotypes decreased. Notably, genotypes Shatabdi, BARI Gom 25, E 18, E 34, E 38, and BAW 1118 exhibited Stress Tolerance Index (STI) values greater than 0.700. In contrast, genotypes BARI Gom 26, E 2, E 23, E 29, BAW 1130, BAW 1140, BAW 1151, BAW 1157, and BAW 1161 displayed STI values lower than 0.550. The genotypes showing higher and lower STI values denoted water stress tolerant and susceptible genotypes, respectively.Öğe Zinc oxide nanoparticles for sustainable agriculture: A tool to combat salinity stress in rice (Oryza sativa) by modulating the nutritional profile and redox homeostasis mechanisms(Elsevier BV, 2025-03) Yusuf Dogan; Pravej Alam; Haider Sultan; Renuka Sharma; Sipan Soysal; Mehmet Firat Baran; Mohammad FaizanThe use of nanoparticles (NPs) as an amendment to reduce salt toxicity has gained much attention. Keeping in mind, this research work was done to evaluate the effect of zinc oxide NPs (ZnO-NPs) to mitigate the salt stress in rice (Oryza sativa) plant. Rice plants were subjected to salt stress (150 mM of NaCl) at 15 days of sowing through the soil. ZnO-NPs were characterized by Scanning electron microscopy (SEM), Transmission electron microscopy (TEM), X-ray diffraction (XRD), and Fourier-transform infrared spectroscopy (FTIR) and were applied foliar at concentration of 100 mg/L for five consecutive days (26–30 DAS). The results confirmed the salt toxicity and reduced shoot length (27 %), root fresh weight (31 %), SPAD chlorophyll (29 %), net photosynthetic rate (24 %), and nitrogen (N), phosphorus (P), potassium (K) and zinc (Zn) uptake by 9 %, 11 %, 13 % and 17 % respectively, while salinity increased the activity of antioxidant enzymes, proline, hydrogen peroxide (H2O2), and malondialdehyde (MDA) content in rice plants. However, in plants grown under salt stress, foliar application of ZnO-NPs significantly improved growth, photosynthesis, nutrient uptake and antioxidant enzymes activity. Beside, ZnO-NPs reduced salinity-induced oxidative stress by lowering H2O2 and MDA content. Therefore, our research showed that ZnO-NPs is useful and efficient in encouraging growth and lessening salinity stress in rice plants.