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    Nitrate and Nitrite Levels in Serum and Seminal Plasma in Horses
    (2018) Aslan, Leyla Mis; Mert, Handan; Söğütlü, İnci Doğan; Pala, Nihat Mert; İrak, Kıvanç
    Nowadays, the fertility of the growing horses is of great importance. The aim of our study is to determine and compare the level of nitric oxide oxidation products of these animals in serum and seminal plasma. Seven healthy British horses were used in the study. Blood samples were taken from the vena jugularis. The sera were removed by centrifugation. Seminal plasmas of ejaculates taken with artificial vagina were separated. Seminal plasma and serum were obtained and nitrate nitrite values were determined. Nitrate and nitrite levels of samples were measured by coupling reagent method. Nitrate levels in both semis and seminal plasma were 6.34 ± 2.7 and 4.95 ± 1.4, respectively. Nitrite levels were higher than seminal plasma in seminal plasma (p? 0.05), while nitrite levels in seminal plasma were higher than serum (p? 0.05). As a result, seminal plasma and serum nitrate-nitrite values in British stallions were used. Presented to the knowledge of clinical veterinary medicine.
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    Öğe
    The Effects of Grape Seed Extract on The Some Enzymes and Metabolites in Diabetic Rats
    (2018) İrak, Kıvanç; Pala, Nihat Mert; Mert, Handan; Ayşin, Nesrullah
    In this study, the effects of Grape Seed Extract (GSE) on glucose, enzymatic activities of elastase and collagenase, and certain biochemical parameters, were investigated. 32 female Wistar albino rats were used in the process. The subjects were randomly divided into 4 groups (control, diabetic (DM), GSE, DM+GSE). A single dose of saline was injected intraperitoneally to the rats in the control group and a single dose of streptozotocin (STZ, 45 mg/kg) was administered intraperitoneally to the rats in the diabetic group. In the GSE group, GSE (0.6 ml/rat) was administered daily via intragastric tube for 20 days, and DM+GSE group received a single dose of STZ intraperitoneally along with 0.6 ml GSE/rat. After 72 hours, blood samples were taken from all the rats. Rats with a fasting blood glucose level of 250 mg/dL and more were considered to be diabetic. At the end of the experiment, blood samples were taken again and were sent for elastase, collagenase, glucose, urea, total protein, cholesterol, and AOPP analysis. Glucose levels in diabetic rats were 671 mg/dL, compared to the glucose level of 335 mg/dL in the DM+GSE group. Total protein levels decreased in the DM+GSE group; the levels of glucose, urea, cholesterol and ascorbic acid also demonstrated a significant difference (p<0.001). No significant change was detected in the AOPP levels. The activities of collagenase (p<0.01) and elastase (p<0.05) in the DM group had moderate increases with respect to the control. It can, therefore, be surmised that the GSE reduces glucose levels, inhibits production of urea, regulates renal function, prevents possible nephropathy, overcomes albuminuria and alleviates protein loss, increases vitamin C levels and minimally affects collagenase and elastase activities.